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자연과학
공학
의약학
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예술체육학
복합학
DBpia AI
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Citeasy
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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 저자정보
초록·키워드
A fibrinolytic enzyme has been found in severalbacteria isolated from fermented food. This study was carriedout to investigate the purification and characteristics of thefibrinolytic enzyme produced by Bacillus subtilis KCK-7originated from Chungkookjang. The fibrinolytic enzymewas purified to homogeneity from the culture supernatantusing ammonium sulfate fractionation and chromatographieson DEAE-cellulose and on Sephadex G-100. The finalspecific activity of the purified enzyme increased 11.0-fold,and the protein amount in the purified enzyme was about 16%of that in the culture supernatant. The molecular weight of thepurified enzyme was estimated to be about 45,000 by SDSPAGE.The optimum pH and temperature for the enzymeactivity were pH 7.0 and 60oC, respectively. The enzymeactivity was relatively stable up to 60oC over the pH range of7.0- 10.0. The fibrinolytic enzyme activity increased by Ca2+and Cu2+, whereas it was inhibited by Hg2+ and Ba2+. Inaddition, it was severely inhibited by PMSF and DFT. Itis suggested that the purified enzyme was a serine proteasefor the fibrinolysis. The purified enzyme could completelyhydrolyze fibrin in vitro within 8 h. Hence, it is suggested thatthe purified enzyme can be put into practice as an effectivethrombolytic agent.
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