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논문 기본 정보
- 자료유형
- 학술저널
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제1호
- 발행연도
- 2014.1
- 수록면
- 48 - 51 (4page)
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α-Neoagarooligosaccharide (α-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular masses of α-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 and 93 kDa, respectively, and the optimal temperature and pH for the enzyme activity were 32ºC and 7.0-7.2. α-NAOS hydrolase lost 43% of its original activity when incubated at 35ºC for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose to galactose, agarotriose, and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly; however, it did not degrade agarose.
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참고문헌 (17)
참고문헌 신청
Ariga, Osamu / 2012 / Cloning of Agarase Gene from Non-Marine Agarolytic Bacterium Cellvibrio sp. / Journal of Microbiology and Biotechnology 22 (9) : 1237 ~ 1244
Chen H / 2006 / Antioxidant activity and hepatoprotective potential of agaro-oligosaccharides in vitro and in vivo / Nutr. J 31 : 1 ~ 12
Chen HM / 2005 / Antioxidant activities of agarooligosaccharides with different degrees of polymerization in cell-based system / Biochim. Biophys. Acta 1722 : 103 ~ 111
Enoki T / 2010 / Oligosaccharides from agar inhibit pro-inflammatory mediator release by inducing heme oxygenase 1 / Biosci. Biotechnol. Biochem 74 : 766 ~ 770
Enoki T / 2012 / Anti-tumor-promoting activities of agarooligosaccharides on two-stage mouse skin carcinogenesis / Biol. Pharm. Bull 35 : 1145 ~ 1149
Chen H / 2006 / Antioxidant activity and hepatoprotective potential of agaro-oligosaccharides in vitro and in vivo / Nutr. J 31 : 1 ~ 12
Chen HM / 2005 / Antioxidant activities of agarooligosaccharides with different degrees of polymerization in cell-based system / Biochim. Biophys. Acta 1722 : 103 ~ 111
Enoki T / 2010 / Oligosaccharides from agar inhibit pro-inflammatory mediator release by inducing heme oxygenase 1 / Biosci. Biotechnol. Biochem 74 : 766 ~ 770
Enoki T / 2012 / Anti-tumor-promoting activities of agarooligosaccharides on two-stage mouse skin carcinogenesis / Biol. Pharm. Bull 35 : 1145 ~ 1149
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