인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 425 - 433 (9page)
- DOI
- 10.7783/KJMCS.2021.29.6.425
이용수
초록· 키워드
Background: Rubus coreanus and R. occidentalis are commonly sold as, ‘Bokbunja’, in the Korean market. However, according to Korean Pharmacopoeia, ‘Bokbunja’ refers to the unripened fruits of R. coreanus. Therefore, authentication of R. coreanus and its related species is necessary to prevent adulteration and improper use.
Methods and Results: Genomic DNA was extracted from leaves, stem and fruits of R. coreanus, fruits of R. occidentalis and leaves of R. longisepalus and R. hirsutus. The anthocyanin profile of R. coreanus and R. occidentalis was identified by high performance liquid chromatography/diode-array detection. We designed four universal barcoding primer sets with optimal restriction enzyme sites to identify polymerase chain reaction-restriction fragment length polymorphisms (PCRRFLPs) between R. coreanus, the newly assembled chloroplast genome of R. occidentalis, and two related species. In addition, two Insertion or Deletion (InDel) markers were also developed. These markers were successfully used to distinguish each species in nine samples.
Conclusions: In this study, we sequenced and assembled the complete chloroplast genome of R. occidentalis and developed a DNA barcoding method to differentiate it from other related species. Thus, anthocyanin profiling, PCR-RFLPs, and InDel markers are useful authentication tools to distinguish R. coreanus from R. occidentalis.
Methods and Results: Genomic DNA was extracted from leaves, stem and fruits of R. coreanus, fruits of R. occidentalis and leaves of R. longisepalus and R. hirsutus. The anthocyanin profile of R. coreanus and R. occidentalis was identified by high performance liquid chromatography/diode-array detection. We designed four universal barcoding primer sets with optimal restriction enzyme sites to identify polymerase chain reaction-restriction fragment length polymorphisms (PCRRFLPs) between R. coreanus, the newly assembled chloroplast genome of R. occidentalis, and two related species. In addition, two Insertion or Deletion (InDel) markers were also developed. These markers were successfully used to distinguish each species in nine samples.
Conclusions: In this study, we sequenced and assembled the complete chloroplast genome of R. occidentalis and developed a DNA barcoding method to differentiate it from other related species. Thus, anthocyanin profiling, PCR-RFLPs, and InDel markers are useful authentication tools to distinguish R. coreanus from R. occidentalis.
#Rubus coreanus
#Chloroplast
#Insertion or Deletion Marker
#PCR-RFLP
#Single Nucleotide Polymorphism
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목차
- ABSTRACT
- 서언
- 재료 및 방법
- 결과 및 고찰
- REFERENCES
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