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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.11
- 수록면
- 138 - 138 (1page)
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초록· 키워드
Psoriasis is a chronic inflammatory skin disease and immune-mediated disease. Several psoriasis animal models have been developed. Sphingolipids have been known to regulate the biological functions and immune cells in skin. In this study, ceramide analysis by LC-MS/MS and inhibition of SphK1 by PF543 in IMQ-induced psoriasis mouse model were investigated.
8-week-old BALB/c mice were housed. The mice were divided into four groups: Vaseline treated control group, imiquimod-treated psoriasis group (IMQ) (3.125 mg/mouse), IMQ plus PF-543 group (1 mg/kg), IMQ plus ethanol group. On the 10<SUP>th</SUP> day, mice were sacrificed to collect plasma, spleen, and skins samples.
The ceramides from plasma and back skin samples were extracted by chloroform: methanol (2:1) mixture. The chloroform phase was dried at a vacuum evaporator and the dry residue was reconstituted in 100 μl MeOH before injecting into LC-MS/MS. The samples were eluted from CAPCELL PAK MG III C18 column with gradient elution with mobile phase A (10 mM ammonium acetate in water with 0.1% formic acid) and mobile phase ... 전체 초록 보기
상세정보 수정요청해당 페이지 내 제목·저자·목차·페이지8-week-old BALB/c mice were housed. The mice were divided into four groups: Vaseline treated control group, imiquimod-treated psoriasis group (IMQ) (3.125 mg/mouse), IMQ plus PF-543 group (1 mg/kg), IMQ plus ethanol group. On the 10<SUP>th</SUP> day, mice were sacrificed to collect plasma, spleen, and skins samples.
The ceramides from plasma and back skin samples were extracted by chloroform: methanol (2:1) mixture. The chloroform phase was dried at a vacuum evaporator and the dry residue was reconstituted in 100 μl MeOH before injecting into LC-MS/MS. The samples were eluted from CAPCELL PAK MG III C18 column with gradient elution with mobile phase A (10 mM ammonium acetate in water with 0.1% formic acid) and mobile phase ... 전체 초록 보기
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