인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
This paper presents a cell electroporationcharacterization chip using a single tapered channelfor a continuous gradient of electric fields. In the cellelectroporation study, it is required to find the optimalelectric field strength for obtaining the maximum num-ber of both viable and electroporated cells possible.
The previous electroporation chip with a single step-wise channel generated a limited number of electricfields. However, the present chip, where continuouselectric fields (0.3-0.5 kV/cm) are generated by a pairof external electrodes across a tapered single channel,provides a more finely tuned electric field for stablecell electroporation with high viability. In the experi-mental study, we characterize H23 non-small-cell lungcancer (NSCLC) cells. In the previous electroporationchip, the percentages of viable electroporated H23 cellswere 51.4% and 44.5% at 0.4 and 0.45 kV/cm, respec-tively. The present chip was measured to have an elec-tric field of 0.44 kV/cm with a maximum percentageof viable electroporated cells of 54.7?4.8%. The pre-sent cell electroporation characterization chip has po-tential for use in integrated cell chips to find fine opti-mal electric field conditions for the study of electro-poration
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