인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 523 - 529 (7page)
이용수
초록· 키워드
In this study, rabbit corneal epithelium was reconstructed using blinking dynamic culture with a selfmanufactured amniotic membrane (AM) supporter composed of three Teflon rings and a lyophilized amniotic membrane (LAM). The reconstructed corneal epithelium was characterized by histological (H & E) and immunohistochemical staining (PCNA) and by RT-PCR and TEM. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the medium supply during the dynamic culture. The cytokeratin 3 (CK3) mRNA expression levels in tilting and blinking dynamic cultured third passage RCE cells seeded onto a LAM were about 2 times greater than that in static culture. The morphology of the corneal epithelium reconstructed on a LAM via blinking dynamic culture over a period of six days better resembled that of the normal cornea than the corneal epithelium reconstructed via the static and tilting dynamic culture. Thus, the reconstruction of the corneal epithelium using a LAM and blinking dynamic culture method is considered to be a good in vitro model for the autologous or allogeneic transplantation of corneal epithelium and it can be quite useful in toxicological test kits.
#simulation
#blinking dynamic culture
#reconstruction
#corneal epithelium
#lyophilized amniotic membrane
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