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Fusobacterium(F) nucleatum, one of the bacteria causing halitosis, produces the, volatile sulfur compounds(VSC) such as H2S in the media containing sulfur components, and forming FeS by binding with iron components in broth. The amount of sulfur compounds produced by F. nucleatum was measured to assess the usefulness of method using spectrophotometer. After F. nucleatum was incubated in the media containing ZnC2 and chlorhexidine dihydrochloride the concentration of sulfur compounds measured by Halimeter was compared with the optical density measured by spectrophotometer.

1. When F. nucleatum was cultured in the closed tube for 24 hours, the concentration of VSC was increased to above 20,000 ppb measured with Halimeter, and the optical density(O.D) was 0.429±040. When being cultured in the open tube, the concentration of VSC was slightly increased to 600±10 ppb, and O.D. to 0.131±0.053.
2. When pH of the media cultured with F. nucleatum for 24 hours was adjusted and incubated for 24 hours, O.D. was about 1.000 in media adjusted to pH 7.0 or 6.5, 0.704 in media adjusted to pH 6.0, 0.295 in media adjusted to pH 5.5, -0.016 in media adjusted to pH 5.0, and -0.119 in media adjusted to pH 4.5.
3. The concentration of VSC, O.D. were above 20,000 ppb, 2.064±0.007 by the addition of 0.03125% ZnCl2 in media, 157±13 ppb, 0.329±0.052 by the addition of 0.25% ZnCl2. The concentration of VSC and O.D. were 5,887±2823 ppb and l.290±0.010 by the addition of 0.0003125% chlorhexidine dihydrochloride, 893±247 ppb and -0.452±0.106 by the addition of 0.02% chlorhexidine dihydrochloride. These results suggested that the sulfur compounds produced by F. nucleatum was similarly quantified by Halimeterⓡ and spectrophotometer, so the measurement of optical density by spectrophotometer was useful in the study of halitosis.

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UCI(KEPA) : I410-ECN-0101-2009-515-013693731