지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Angiogenesis has been implicated in progression of inflmmation, arthritis, psoriasis, atherosclerosis as well as tumor growth and metastasis. Intensive studies have been carried out to develop a strategy for cancer treatment by blocking angiogenesis. During angiogenesis, endothelial proliferation and migration essentially occurs upon activation. In this study, we compared the expression profiles of human umbilical endothelial cells activated by incubating in vitro in the rich medium containing several growth factors, and non-activated ones. cDNA targets derived from total RNAs of HUVEC activated for 13 h in M199 medium containing endothelial cell growth supplement, 20% fetal bovine serum, and heparin, after reaching 70～80% confluency, or non-activated, were hybridized onto oligonucleotide microarrays containing 1,8864 genetic elements. Unsupervised hierarchical clustering analysis resulted in two subgroups on dendrogram exhibiting activated and non-activated HUVECs. We then extracted 122 outlier genes which were shown to be up-regulated or under-expressed by at least 2-folds in activated HUVECs. Among these, 32 annotated genes were up-regulated and 38 were down-regulated in activated HUVECs. Interestingly, genes involved in cell proliferation, motility, and inflammation/immune response were up-regulated in activated HUVEC, whereas genes for cell adhesion or vessel morphogenesis/function were down-regulated. Unexpectedly, the expression of genes well-characterized as angiogenesis markers was not changed except Eph-B4, which was down-regulated about 4 folds. 52 unknown genes were also up- or down-regulated. Therefore, these results could provide an opportunity to targeting new vascular molecules for the development of anti-angiogenic molecules.
목차
Abstract
재료 및 방법
결과
고찰
요약
감사의 글
참고문헌
참고문헌 (20)
참고문헌 신청
[학술지(정기간행물)] - / 2001 / cDNA microarray analysis of the gene expression profile of VEGF-activated human umbilical vein endothelial cells 4 : 289 ~ 298
[학술지(정기간행물)] - / 1999 / Roles of ephrinB ligands and EphB receptors in cardiovascular development demarcation of arterial/venous domains 13 : 295 ~ 306
[학술지(정기간행물)] - / 1999 / VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cells 18 : 3964 ~ 3972
[학술지(정기간행물)] - / 1997 / Isolation of putative progenitor endothelial cells for angiogenesis 275 : 964 ~ 967
[학술지(정기간행물)] - / 1999 / Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization 85 : 221 ~ 228
[학술지(정기간행물)] - / 1999 / Roles of ephrinB ligands and EphB receptors in cardiovascular development demarcation of arterial/venous domains 13 : 295 ~ 306
[학술지(정기간행물)] - / 1999 / VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cells 18 : 3964 ~ 3972
[학술지(정기간행물)] - / 1997 / Isolation of putative progenitor endothelial cells for angiogenesis 275 : 964 ~ 967
[학술지(정기간행물)] - / 1999 / Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization 85 : 221 ~ 228
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
Experimental and Molecular Medicine
2016 .01
-
Interleukin-2 Promotes Angiogenesis by Activation of Akt and Increase of ROS
Journal of Microbiology and Biotechnology
2008 .01
-
A standardized bamboo leaf extract inhibits monocyte adhesion to endothelial cells by modulating vascular cell adhesion protein - 1
Nutrition Research and Practice
2013 .02
-
A standardized bamboo leaf extract inhibits monocyte adhesion to endothelial cells by modulating vascular cell adhesion protein - 1
Nutrition Research and Practice
2013 .02
-
Functional Classification of Gene Expression Profiles During Differentiation of Mouse Embryonic Cells on Monolayer Culture
Animal cells and systems
2009 .01
-
Global Analysis of Gene Expression upon Acid Treatment in Arabidopsis thaliana
The Plant Pathology Journal
2009 .06
-
Statistical Analysis of Gene Expression Data
한국생물정보시스템생물학회 워크샵
2001 .01
-
Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells
Experimental and Molecular Medicine
2005 .01
-
In Silico Screening for Angiogenesis-Related Genes in Rat Astrocytes
Genomics & informatics
2004 .01
-
Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression
Animal cells and systems
2010 .01
-
Gene Expression Analysis of Gα Knockout Mouse Embryos Reveals Perturbations in Gα Signaling Related to Angiogenesis and Hypoxia
Genomics & informatics
2011 .01
-
청국장 에탄올 추출물의 혈관내피세포 증식과 이동 촉진효과
미생물학회지
2014 .09
-
프마이크로어레이 데이터의 유전자 집합 및 대사 경로 분석
유전체소식
2006 .01
-
Gene network and canonical pathway analysis in prostatecancer: a microarray study
Experimental and Molecular Medicine
2008 .01
-
DNA microarray analysis of gene expression regulated by estrogen in the rat uterus
한국동물학회 학술대회
2001 .01
-
재대정맥 내피세포의 증식에 미치는 글루타민 및 혈청 결핍의 영향
생명과학회지
2013 .07
-
Development of an angiogenesisfocused cDNA chip and validation of its functionality
Experimental and Molecular Medicine
2005 .01
-
Analysis of Gene Expression upon DNA Damage in Arabidopsis
식물학회지
2006 .01
-
Extract of Balloon-flower Inhibited In Vitro Angiogenesis in Human Umbilical Vein Endothelial Cells
생명과학회지
2017 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-470-014448909