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Using a reversed-phase high performance liquid chromatography, the separation of 20(S)-, 20(R)-prosapogenin stereo-isomers of ginsenoside-Rg₂ and of ginsenoside-Rg₃ in ginseng saponins has been carried out with binary solvent system. The optimum conditions for the isomer separation are as following: Nova-Pak®C_(18) (Waters, 3.9×150 ㎜) column, CH₃CN/CH₃CN (100:8, v/v) binary solvent system and the flow rate was 1.7 mL/min. The stereoisomers were separated with change of the mixture ratio of the solvent system, the solvent elution by gradient program, and then detected at 203 ㎚ of UV detector. The simultaneous separation of mixture that were the Rg₂, Rg₃ isomers was easily performed in nonpolar solvent for Rg₂, polar solvent for Rg₃ at the same optimum conditions.
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Abstract
1. 서론
2. 실험
3. 결과 및 고찰
4. 결론
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UCI(KEPA) : I410-ECN-0101-2009-433-018129015