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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국수산과학회 양식분과 한국양식학회지 한국양식학회지 제8권 제4호
발행연도
1995.11
수록면
295 - 306 (12page)

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The present study was performed to produce supermales and superfemales in Oreochromis niloticus by combination of induced sex reversal and diploid gynogenesis.
More than 95% of female was obtained by oral administration of 17β-estradiol (480 mg/kg diet) to the eutheroembryonic larvae of this species. The result of progeny tests with XY pseudofemales showed that incidences of male progeny were ranged from 71.4% to 73.7%. Proportion of male from XY pseudofemale was not significantly different (P>0.05) from that of X² (1 : 3), but it was significantly different (P<0.01) from that of X² (1 : 1). Gynogenetic diploids were produced by applying cold shocks 3 min after insemination to the eggs of XY pseudofemale sperms were genetically inactivated by ultraviolet rays of 4,050 erg/㎟. Fertilization rates of gynogenetic diploids were not different from that of their controls, however, hatching rates and early survival rates were silghtly lower than those of controls. In the cytogenetic studies, there was no difference in the size of cell and nucleus between the gynogenetic diploids and the controls. At 80 days after hatching, frequencies of gynogenetic male were higher than those of gynogenetic female (P<0.01) in their population.

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