메뉴 건너뛰기
소속 기관 / 학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
고객센터 ENG
주제분류

논문 기본 정보

저자정보
저널정보
한국수산과학회 양식분과 한국양식학회지 한국양식학회지 제10권 제4호
오류 신고하기

피인용 3

검색

    초록·키워드

    The carp β-actin regulatory sequences and RSV/LTR promoter were tested whether they are functional to express linked structure gene (chloramphenicol acetyltransferase, CAT) in olive flounder (Paralichthys olivaceus) by determining the patterns of gene expression following intramuscular in vivo direct injection. The injection experiments with various concentrations of both pRSVCAT and pFV4CAT clearly revealed the effectiveness of DNA dosage on expression of CAT. The increase of CAT activity was linear in both plasmids, and maximal CAT activity was obtained with 100 ㎍ of pFV4CAT injection. The amounts of CAT expression with pFV4CAT-injected fish were higher than those with pRSVCAT-injected fish. CAT activity was readily detectable as early as one day after injection, slightly increased at day 2, and declined over time. Most amount of DNA intramuscularly injected into olive flounder muscles persisted extrachromosomally without showing any integrated or replicated form in vivo.

    최근 본 자료 전체보기

      UCI(KEPA) : I410-ECN-0101-2009-529-015350064