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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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In previously study, we isolated several fish matrix attachment regions (MARS) capable of replicating the plasmid by itself. In this study, we construct a fish expression vector, pBaEGFP(+), containing mud loach β-actin promoter, EGFP as reporter gene, and SV40 poly A signal. To analyze the effects of matrix-attached ARS elements, the cloned ARS elements were inserted into the multicloning site of the fish expression vector respectively. The fish ARS containing constructs, pBaEGFP(+)-ARSs, were transfected into a fish cell line, BF-2. In long-terms expression in BF-2 cells, The intensity of EGFP in transfected cells with pBaEGFP(+)-ARS101 and pBaEGFP(+)-ARS223 reduced 10 days to 25 days and then was constant to 30 days after transfection, while that of the control vector without ARS element was basal level. The intensity of both constructs showed about 3-fold of the intensity compared with the control vector on 30 days after transfection individually. E. coli back-transformation analysis shows that pBaEGFP(+)-ARS223 and pBaEGFP(+)-ARS905 maintain in episomal state at least 30 days after transfection. The result indicates that both may be able to replicate the vector in BF-2 cell. Therefore, the matrix-attached ARSs enhancing expression of the reporter gene might be useful as a component of the expression vector for transgenic studies.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgement
REFERENCES
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UCI(KEPA) : I410-ECN-0101-2009-529-015347181