지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Nebulin is a (Mr 600~900 kDa) large actin-binding protein specific to skeletal muscle and thought to act as a molecular template that regulates the length of thin filaments. Cardiac muscles of higher vertebrates have been shown earlier to lack nebulin. Recently, full-length nebulin mRNA transcripts have been detected in heart muscle, but at lower levels than in skeletal muscle. Nebulin expression also was detected in the kidney, eye, and otic canal, suggesting that nebulin isoforms may also be expressed in these organs. We have searched for nebulin isoforms in brain of human using PCR and Northern blot. Here, we provide evidence that nebulin mRNA transcripts are expressed in brain. Seven nebulin isoforms (B, C, D, E, F, G and H form) are obtained in human skeletal muscle and four isoforms (B, C, G and H form) in human brain cDNA library. We cloned the 1.3 kb of nebulin fragment from human adult brain library by PCR. The identity of the PCR product was confirmed by sequence analysis. The partial brain nebulin sequence was 99% identical to the skeletal muscle cDNA as determined by Blast alignment. It contains two simple-repeats HR1, HR2 and linker-repeats exon 135~143 except exon 140. It was different from skeletal muscle B form, which contain HR1 and HR8. These data suggest that nebulin isoform diversity occurs even more extensively than previously known, likely contributing to the distinct thin filament architecture of different striated muscles.
목차
영어초록
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (31)
참고문헌 신청
[학술지(정기간행물)] - Bang ML, / 2001 / The complete gene sequence of titin, expression of an unusual (70-kDa) titin isoform, and its interaction with obscurin identify a novel Z-line to I-band linking system. 89 : 1065 ~ 1072
[학술지(정기간행물)] - Bang ML / 2002 / Molecular dis- section of the interaction of desmin with the C-terminal region of nebulin. 137 : 119 ~ 127
[학술지(정기간행물)] - Bang ML, / 2001 / Myopalladin, a novel 145-kilodalton sarco- meric protein with multiple roles in Z-disc and I-band protein assemblies. 153 : 413 ~ 427
[학술지(정기간행물)] - Clark KA / 2002 / Striated muscle cytoarchitecture: an intricate web of form and function. 18 : 637 ~ 706
[학술지(정기간행물)] - Chen MJ / 1993 / nebulin is an actin-binding length regulating template protein of the thin fila- ments of skeletal muscle actin-interaction and confirmation of a two module human nebulin fragment. Biophys J : 147 ~ 159
[학술지(정기간행물)] - Bang ML / 2002 / Molecular dis- section of the interaction of desmin with the C-terminal region of nebulin. 137 : 119 ~ 127
[학술지(정기간행물)] - Bang ML, / 2001 / Myopalladin, a novel 145-kilodalton sarco- meric protein with multiple roles in Z-disc and I-band protein assemblies. 153 : 413 ~ 427
[학술지(정기간행물)] - Clark KA / 2002 / Striated muscle cytoarchitecture: an intricate web of form and function. 18 : 637 ~ 706
[학술지(정기간행물)] - Chen MJ / 1993 / nebulin is an actin-binding length regulating template protein of the thin fila- ments of skeletal muscle actin-interaction and confirmation of a two module human nebulin fragment. Biophys J : 147 ~ 159
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Desmin Binding Property of Nebulin Isoforms
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닭 조직에 따른 Nebulin Isoform 단백질의 특이적 발현
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Osteonectin Interacts with Human Nebulin C-terminus in Skeletal Muscle
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Nebulin C-terminus Interacts with NCBP51, a New Isoform of RING Finger Protein 125 (RNF125)
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Regulated Expression of Nebulin by Transfection of Green Fluorescent Protein-Tagged Nebulin Fragments in Cultured Chicken Myoblast
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Identification of a Protein Interacting with Human Nebulin SH3 Domain by Yeast Two-hybrid Screening
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Nebulin in Z-discs and Costameres
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BASIC SCIENCE OF THE SKELETAL MUSCLES
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Cloning of TLR3 Isoform
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Knockdown of Archvillin by siRNA Inhibits Myofibril Assembly in Cultured Skeletal Myoblast
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The Clinicopathological and Prognostic Impact of 14-3-3 Protein Isoforms Expression in Human Cholangiocarcinoma by Immunohistochemistry
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골격근 섬유의 수축성 특성의 다양성
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UCI(KEPA) : I410-ECN-0101-2009-510-017341393