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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.12
- 수록면
- 487 - 492 (6page)
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The baculovirus / insect cell expression system is of great value in the study of structure-function relationships in mammalian glucose-transport proteins by site-directed mutagenesis and for the large-scale production of these proteins for. mechanistic and biochemical studies. Spodoptera frugiperda Clone 21 (Sf21) cells grow well on TC-100 medium that contains 0.1% D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transporters. However, very little is known about the properties of the endogenous sugar transporter(s) in Sf21 cells, although a saturable transport system for hexose uptake has been previously revealed in the Sf cells. In order to further examine the substrate and inhibitor recognition properties of the Sf21 cell transporter, the ability of hexoses to inhibit 2-deoxy-D-glucose (2dGlc) transport was investigated by measuring inhibition constants (K<sub>i</sub>). The K<sub>i</sub>'s for reversible inhibitors were determined from plots of uptake versus inhibitor concentration. Transport was effectively inhibited by D-mannose and D-glucose. Of the hexoses tested, L-glucose had the least effect on 2dGlc transport in the Sf21 cells, indicating that the transport is stereoselective. Unlike the human HepG2 type glucose transport system, D-mannose had a somewhat greater affinity for the Sf21 cell transporter than D-glucose, implying that the hydroxy1 group at the C-2 position is not necessary for strong binding. However, epimerization at the C-4 position of D-glucose (D-galactose) resulted in a dramatic decrease in affinity of the hexose far the Sf21 cell transporter. Such a lowering of affinity might be the result of the involvement of the C-4 hydroxyl in hydrogen bonding. It is therefore suggested that Sf21 cells were found to contain an endogenous sugar transport activity that in several aspects resembles the human HepG2 type glucose transporter, although the insect and human transporters do differ in their afnity for cytochalasin B.
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Hexose Uptake and Kinetic Properties of the Endogenous Sugar Transporter(s) in Spodoptera frugiperda Clone 21-AE Cells
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Effects of Phloretin, Cytochalasin B, and D-Fructose on 2-deoxy-D-Glucose Transport of the Glucose Transport System Present in Spodoptera frugiperda Clone 21-AE Cells
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Effects of Pentoses on 2-deoxy-D-Glucose Transport of the Endogenous Sugar Transport Systems in Spodoptera frugiperda Clone 9 Cells
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2009 .03
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The Uptake of 2-deoxy-D-glucose (2dGlc) by the Endogenous Sugar Transporter(s) of Spodoptera frugiperda CLone 21-AE Cells and the Inhibition of 2dGlc Transport in the Insect Cells by Fructose and Cytoc halasin B
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Cross-reactivity of Human Polyclonal Anti-GLUT1 Antisera with the Endogenous Insect Cell Glucose Transporters and the Baculovirus-expressed GLUT1
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Tryptic Digestion and Cytochalasin B Binding Assay of the Human HepG2-Type Glucose Transporter Expressed in spodoptera frugiperda Clone 21-AE Cells
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Photoaffinity Labelling of the Human Erythrocyte Glucose Transporters Expressed in Spodoptera frugiperda Clone 9 (Sf9) Cells
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Toxicological Relevance of Transporters
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Subcellular Location of Spodpotera Cell-expressed Human HepG2-type Glucose Transport Protein
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Hydrogen Peroxide Mediates Brazilin-induced Glucose Transport in Adipocytes
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High Glucose Inhibitory Effects on Bone Formation in UMR106 Cells
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