黃色 모자이크, 壞疽條斑, 委縮, 寄形, colour breaking의 病微을 나타내고 있는 나리類를 韓國의 南部地域에서 採集하였다. 電子顯微鏡에 의해 2種의 바이러스 粒子가 分離되어, 이들의 寄主範圍, 血淸反應, 粗汁液中의 不活化 限界, 진딧물의 傳搬性, 感染葉細胞에 있어서 바이러스 粒子의 所在樣式에 의하여 2種의 바이러스를 同定 하였다. Broad bean wilt virus(BBWV) : 이 바이러스는 汁液接種에 의해 8科 23種의 檢定植物에 感染이 확인되었다. 복숭아흑진딧물에 의해 쉽게 傳搬되었다. 耐熱性은 70℃, 耐稀釋性은 1,000倍, 耐保存性은 6日이였다. 바이러스 粒子의 形態는 直徑 約 28㎚의 小球形이다. 血淸試驗에서 本 바이러스는 BBWV의 抗血淸과 特異的인 反應帶를 形成했다. 病葉切片의 電子顯微鏡的觀察에서는 葉肉細胞의 細胞質, 液胞, 核內에서 바이러스 粒子의 凝集塊 및 結晶配列像이 확인되었고, 특히 細胞質內에서는 vesicular body의 發達이 확인되었다. Cncumber mosaic virus(CMV) : 이 바이러스 粒子의 形態는 直徑 約 30㎚의 小球形이다. 本 바이러스는 CMV-Y의 抗血淸과 特異的인 反應帶를 形成했다. 感染葉組織의 電子顯微鏡的觀察에서는 葉肉細胞의 細胞質 및 液胞內에서 바이러스 粒子의 凝集塊 또는 結晶配列像이 확인되었다. BBWV와 CMV를 건전한 나리類(Lilium tigrium, L. concolor, L. auratum)에 再接種한 結果, BBWV의 接種株에서는 가벼운 病徵 또는 colour breaking를 나타내었고, CMV의 接種株에서는 黃色 모자이크 또는 壞疽條斑을 나타냈다.
Samples showing yellow mosaic symptom of Lilium spp. with necrotic fleck, stunting, malformation, and colour breaking were collected from lily-growing areas in the southern part of Korea. Two viruses were distinguished under a electron microscope and their host range, serological reaction, stability in sap, type of aphid transmission, and relations with cells and tissues were examined. Broad bean wilt virus (BBWV) was transmitted by sap-inoculation to 23 plant species in 8 families and by the aphid, Myzus persicae. This virus was inactivated after 10 min at 70C, at dilution of 10?³, and after 6 days at about 20C. Electron microscopic examination of purified preparation showed that the virus is spherical particle of 28㎚ in diameter. The virus reacted positively with BBWV-antiserum in agar gel diffusion test. In ultrathin sections of BBWV infected tissues, large aggregates or crystalline array of virus particles and vesicular body were found in the cytoplasm, vacuole, and nucleus of mesophyll cells. Cucumber mosaic virus (CMV) was transmitted by sap-inoculation. Electron microscopic examination of its purified preparation showed spherical particles of 30㎚ in diameter. The virus reacted positively with CMV-Y strain-antiserum in agar gel diffusion test. In ultrathin sections of CMV infected tissues, crystalline array of virus particles were found in the vacuole and a large number of virus particles were found in the cytoplasm and the plasmodesmata of mesophyll cells. When each of these viruses was retransmitted to Lilium tigrinum, L. concolor, and L. auratum, BBWV induced slight symtoms and colour breaking, but CMV induced yellowing mosaic or necrotic fleck.