시설재배지 토양으로부터 분리된 167균주 중 딸기눈마름병균인 Rhizoctonia solani AG2-1에 대한 길항 효과를 나타내는 40개 균주를 in vitro test에서 선발하였다. 이들 길항균주의 딸기눈마름병균에 대한 생물적 검정을 배추유묘를 사용하여 조사한 결과,Kr013과 Kr020의 2개 균주가 발병억제효과를 보였다. 또한 이들 2개 균주의 탄립매체(charcoal carrier granule)를 딸기유묘 이식구멍에 처리하여도 발병억제 효과를 나타내었다. 한편 Kr013과 Kr020균주 각각의 탄립매체를 20% 첨가한 상토에서 딸기자묘(runner)를 20일간 육묘한 다음 병원균 오염토양에 정식할 경우에는 발병율이 각각 7.3%, 5.7%로서, 발병율이 각각 16.7%와 15.7%를 보인 정식직전에 이들 균주의 탄립매체를 토양처리한 것 보다 높은 방제효과를 보였다. 그리고 생물적 방제효과를 나타낸 Kr013과 Kr020의 두 균주를 Biolog GN Microplate System을 이용하여 동정한 결과, 유사성이 각각 55%와 60%의 Pseudomonas cepacia로 동정되었다.
Forty microbial isolates out of 167 isolates from the soil of controlled cultivation areas inhibited mycelial growth of Rhizoctonia solani AG2-1 causing the strawberry bud rot in vitro. Among the isolates, Kr013 and Kr020 showed suppressive effect to R. solani AG2-1 on seedlings of chinese cabbage treated by root immersion, charcoal carrier granule and drenching on 1.0% infested soil in pot. Furthemore, the corresponding effect was also revealed when the charcoal carrier granule of the isolates were treated on the seedling of strawberry that were planted on the planting hole in pot. To examine the effects of biological control in green house, it had been tested the infection rates by using two different treatments. First, the strawberry runner were planted on the nursery soil mixed with 20% charcoal carrier granule of Kr013 and Kr020 isolate respectively, and grown for 20 days before transplanting. Then the young plants from the mother plant were separated and transplanted on the 1.0% infested soil. Another method was that the charcoal carrier of Kr013 and Kr020 isolates applied to planting hole of 1.0% infested soil just before transplanting. Then the young plants were grown for 20 days on the sterilized nursery soil before transplanting. From the results, the effects of biological control was significantly higher on former treatment (e.g, the infection rates were 7.3 and 5.7%, respectively) than on the latter treatment (e.g, the corresponding value were 16.7 and 15.7%, respectively). The antagonistic isolates of Kr013 and Kr020 were respectively identified as Pseudomonas cepacia with the similarity of 55.0% and 60.0% by using the Biolog GN Microplate system.