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자료유형
학술저널
저자정보
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.15 No.5
발행연도
1999.10
수록면
280 - 286 (7page)

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초록· 키워드

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Lichen-forming (LFF) or lichenicolous fungi (LCF) were isolated from the lichens collected at ‘Backwoon’ mountain area, ‘Chiri’ mountain area and ‘Sorok’ island in the southern regions of Korea and were screened for antagonistic efficacy against several phytopathogenic fungi. Symbiotic algae-free LFF and LCF were isolated by the following methods: Ⅰ) discharged spores (ascospores), Ⅱ) macerated thallus suspension and Ⅲ) direct use of thallus fragments. Among 58 isolates obtained from 34 lichens, 8 isolates showed antifungal activity against Rhizoctonia solani. Antifungal activities of the strongest antagonistic isolate (LB9810) originated from the thallus of Parmelia quercina lichen were evaluated against 15 phyto-pathogenic fungi. When crude methanol extract of mycelia of the LB9810 isolate was employed at the rate of 0.5% (v/w), fungal growth of Magnaporthe grisea and Rhizoctonia solani was severely inhibited as much as approximately 60% compared to control. Growth of various food-borne bacterial pathogens was also severely inhibited by the same extract. The extract was successively partitioned with n-hexane, ethyl acetate and n-butanol. n-Hexane fraction displayed the strongest antifungal activities against R. solani. The LB9810 isolate was finally identified as Fusarium equiseti (Corda) Sacc., which has not been reported as LFF or LCF yet. Therefore, it is very likely that F. equiseti isolated in this study was originated from the contaminants associated with thallus fragments rather than from LFF or LCF.

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Materials and Methods
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