지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1999.6
- 수록면
- 152 - 157 (6page)
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초록· 키워드
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An antibiotic compound was isolated from the culture of an antagonist against Fusarium oxysporum f. sp. sesami, Bacillus polymyxa strain KB-8, and tested for the control of Fusarium wilt of sesame in greenhouse conditions. Optimum conditions for culturing the antagonist to obtain the maximum antibiotic activity were determined using different culture media, initial medium acidity, and incubation periods, for which yeast-malt extract agar with the initial acidity of pH 5 and over 13 days culture were best. Antibiotic substances extracted by methanol had 2 main fractions, KB-8A and KB-8B, in thin layer chromatography (TLC) with Rf values of 0.35 and 0.67 in a solvent system of chloroform : methanol=7:3. The fraction KB-8A was purified further by XAD-2, silica gel and Sephadex LH-20 column chromatography, and crystalization. Its minimum inhibitory concentrations (MICs) were 12.8 ㎍/ml for F. oxysporum and Alternaria mali, 6.4 ㎍/ml for Colletotrichum gloeosporioides and Rhizoctonia solani, and 3.2 ㎍/ml for Phytophthora capsici. Soil drenching of antibiotic KB-8A in the concentrations of 13.0 ㎍/ml and 26.0 ㎍/ml effectively inhibited the Fusarium wilt of sesame in a greenhouse test, which appeared to be comparable to the fungicide benlate of 6.5 ㎍ a. i/ml.
목차
Materials and Methods
Results
Discussion
References
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UCI(KEPA) : I410-ECN-0101-2009-481-017560254