고추炭疽病菌 Colletotrichum gloeosporioides Penz. R系統에 對한 푸른고추의 抵抗性機作을 糖, 蛋白質, 페놀化合物 및 Peroxidase 活性과 原形質模의 損傷을 연관지어 考察하였다. R系統은 푸른고추열매의 生體에서는 菌叢을 形成하지 못했으나 高溫處理와 冷凍處理한 고추열매에서는 菌叢을 形成하였다. 푸른고추와 붉은고추 抽出物로 만든 寒天培地上에서 R系統의 菌絲成長은 差異가 없이 잘 자랐다. 푸른고추열매內의 蛋白質 및 總페놀의 含量 및 組成은 高溫處理, 冷凍處理 및 生體內에서 差異가 認定되지 않았다. Peroxidase 活性은 生體와 冷凍處理한 고추열매間에는 差異가 없이 높았으나 高溫處理한 열매에서는 전혀 活性이 없었다. R系統을 接種한지 48時間된 羅病열매와 健全열매에서 蛋白質, 總페놀 含量 및 Peroxidase 活性을 調査, 比較한 結果 그 差異는 認定되지 않았다. 高溫處理와 冷凍處理한 푸른고추열매組織漏出物에서 糖 , 蛋白質含量 및 電解質傅導度는 時間이 經過함에 따라 크게 增加하였으며 生體의 열매組織에서의 漏出物은 모두 거의 增加가 없었다. 따라서 植物體의 羅病化는 原形質模의 損傷과 관계가 있는 것으로 思料된다.
The present studies were carried out to investigate the changes of total sugar, protein, phenolic content and peroxidase activity in green pepper (Capsicum annuum L.) in relation to disease resistance to anthracnose fungus, Colletotrichum gloeosporioides R strain. The fungus could not colonize the fresh green pepper fruist, but colonize the autoclaved or the frozen green pepper fruits. The mycelia of R stranin could grow well on green pepper extract agar and on red pepper extract agar as well as on potato sucrose agar. No difference in protein and total phenol contents was found among the fresh, autoclaved, and frozen fruits of green pepper. There was no consistent difference in peroxidase activity between fresh and frozen fruits, but such enzyme activity was not found in autoclaved fruits. Composition of phenolic compounds was same in fresh, frozen and autoclaved fruits of green pepper on thin layer chromatography. There were no differences in protein, total phenol content and peroxidase activity between healthy and infected fruits of green pepper. The leakage of carbohydrate, protein, and electrolyte markedly increased from autoclaved and frozen tissue of green pepper fruits, but did not increase from fresh tissue.