지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.9
- 수록면
- 288 - 292 (5page)
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초록· 키워드
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Pathogenesis-related protein-1a (PR-1a) is strongly induced in tobacco plants by pathogen attack, exogenous salicylic acid (SA) application and by other developmental processes. In order to develop a rapid screening system for the selection of plant defenseinducing compounds originated from various sources, we have transformed tobacco Samsun NN plants with a chimeric construct consisting of GUS(β-glucuronidase). In the T₁ generation, three transgenic lines having stable GUS expression were selected for further promoter analysis. Using GUS histochemical assay, we observed strong GUS induction driven by PR-1a promoter in PR1a-GUS transgenic tobacco leaves in response to the exogenous application of SA or benzol (1, 2, 3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), a SA-derivative compound. In addition, GUS expression was maintained locally or systemically in PR1a-GUS transgenic line # 5 (T₂ generation) until after 3 days when they were treated with same chemicals. Our results suggested that the PR1a-GUS reporter gene system in tobacco plants may be applicable for the large-scale screening of defense-inducing substances.
목차
Materials and Methods
Results and Discussions
Acknowledgements
References
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UCI(KEPA) : I410-ECN-0101-2009-481-017570752