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자료유형
학술저널
저자정보
저널정보
한국생명과학회 생명과학회지 생명과학회지 제16권 제7호
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1,141 - 1,147 (7page)

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초록· 키워드

This study was carried out to establish a reproducible culture system for callus formation and adventitious shoot development from young stem segments of Vigna radiata, and histological work for orgin of callus tissue and adventitious shoot. Induction of callus from young stem explants of Vigna radiata was very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. For the adventitious shoot regeneration from the callus tissues, the hormone combination of 0.75 mg/L NAA, 1.5 mg/L kinetin and MS salts resulted in about 21% efficiency. Histological examination showed that callus tissues originated from out-growths by callus cambium rings with de novo meristematic activities, which were localized at the outside of the vascular cambium. Adventitious shoots were developed from shoot apical meristem originated from the surface of callus masses. The shoot apical meristem produced leaf primordium, which then became leaf.
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  3. 고찰
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UCI(KEPA) : I410-ECN-0101-2009-470-017616923