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Selection of Species-specific RAPD Markers and Genetic Relationships among Prunus Taxa 핵과류 종 특이 RAPD 표지 선발과 유연관계 분석

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Selection of Species-specific RAPD Markers and Genetic Relationships among Prunus Taxa
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    초록·키워드

    RAPD analysis was conducted to develop species-specific markers and to establish the genetic relationships among 31 Prunus taxa including P. armeniaca, P. cerasifera, P. domestica, P. mandshurica, P. mume, P. salicina, and plumcot. Of the 342 10-mer random primers tested, 30 primers revealed 46 species-specific markers for the majority of Prunus species. However, no species-specific RAPD markers for P. salicina and plumcot were found. From the amplifications with 85 primers showing polymorphism among taxa, 271 polymorphic RAPD bands were detected. Jaccard’s similarity coefficients were calculated by using the coded data matrix of these bands and then cluster analysis by minimum spanning tree method of NTSYS-PC (Ver. 1.7) was carried out. Thirty-one Prunus taxa were clearly divided into 3 large clusters plums-plumcots, apricots and mumes groups at the level of Jaccard’s similarity coefficient of 0.6. These results were consistent with those generated by the existing classification system. Plumcots have much closer genetic affinity with plums rather than apricots or mumes. Mumes also had closer affinity with plums but not with apricots. The results indicate that RAPD analysis is a useful tool for classification of Prunus taxa and the development of species-specific markers.

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      UCI(KEPA) : I410-ECN-0101-2009-525-016872870