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Alternaria blotch (Alternaria alternata (Fr.) Keissler) in apple production is one of the most serious diseases in Korea. For the identification and early selection of resistant genotypes by using molecular markers, one hundred seedlings of an FJ progeny from susceptible 'Malus domestica cv. Sekaiichi' × resistant 'M. baccata local cv. Jeongseon' were used for developing amplified fragment length polymorphism (AFLP) markers. Evaluation of resistance to Alternaria blotch was conducted by bioassay of AM-toxin, as a substitute for pathogen inoculation. Progeny phenotypes were segregated into 1:1 between resistant and susceptible seedlings. Resistance to Alternaria blotch is known to be controlled by a single recessive gene and cultivars containing homozygous recessive genes show resistance to disease. A total of 256 primer combinations of 16 EcoRI primers and 16 MseI primers were used to screen four DNA samples ('Sekaiichi', 'Jeongseon', resistant bulk, and susceptible bulk) and individuals. Consequently, two putative linked AFLP markers were observed, which appeared in 'Sekaiichi' and the susceptible bulk, but neither in 'Jeongseon' nor in the resistant bulk. The polymerase chain reaction products of EaggMcct-312 and EacgMcct-531 cloned into pGEM-T Easy Vector were sequenced, but three EaggMcct-312 fragments were directly sequenced. Nucleotide sequence analysis of EacgMcct-531 showed homology with MIF4G (middle domain of eIF4G) in Arabidopsis thaliana and Oryza sativa to some degrees by the lowest E-value (E = 3e-38, 3e-37).

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Abstract
Introduction
Materials and Methods
Results and Discussion
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2009-525-016949465