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초록· 키워드

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This study was aimed at determining the genetic relationships among the most important Bougainvillea cultivars grown at the National Botanical Research Institute, Lucknow, India using random amplified polymorphic DNA (RAPD) technology. Fifty random decamer primers were screened and ten were selected for final RAPD analysis. Each primer produced a unique set of amplification products ranging in size from 300 to 2500 bp. The number of bands for each primer varied from 10 in P2 to 18 in P10. These ten primers used in this analysis yielded 167 scorable bands with an average of 11.3 bands per primer. Of the 167 fragments scored from these primers, 26 were monomorphic and 141 were polymorphic (84.4%). The generated similarity matrix showed that the genetic diversity within the tested genotypes was high (average similarity index = 30.1%). Similarity values among the studied genotypes ranged between 6 to 89%. The resulting dendrogram divided the cultivars into two main clusters. The first contained 37 cultivars and was divided into two subclusters at a similarity value of 0.03 with 21 and 16 cultivars in each subcluster, respectively. Subcluster Ⅰ included those cultivars which had affinity with Buttiana group. Subcluster Ⅱ contained cultivars of both Spectabilis and Peruviana groups. The second major cluster contained 55 cultivars, contain species of Glabra group and cultivars whose origin are not well recorded. The similarity data obtained in this study agree to some extent (at least for the Buttiana group) with the previous classification of the tested genotypes. The information obtained from this work may be useful for better management, identification of accessions and also in avoiding duplications or mislabeling of the genotypes studied.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Aknowledgements
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2009-525-016949667