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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.48 No.3
발행연도
2007.6
수록면
165 - 170 (6page)

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To establish an efficient embryogenic culture and regeneration system in ‘Miyagawa Wase’ satsuma mandarin (Citrus unshiu Marc.), several factors affecting embryogenic culture maintenance and plant regeneration were investigated. The highest multiplication of embryogenic culture was obtained on culture media supplemented with 5% sucrose, regardless of medium type. When fractioned by Percoll gradient, the callus collected from the layer of 40% showed higher embryogenesis capacity than the others. Agar concentration and carbohydrate source in the medium also affected somatic embryogenesis and the highest embryogenesis was obtained at 1.4% agar and 7% lactose. The highest cotyledon development from somatic embryos was observed by supplement of 0.7 ㎎ㆍL?¹ adenine into the medium. The normal shoots could be regenerated from the section culture of abnormal cotyledons on the medium containing 1-naphthaleneacetic acid (NAA) and N?-benzyladenine (BA). The most effective concentrations of NAA and BA were 0.01 and 0.1 ㎎ㆍL?¹, respectively. The normal plants were obtained through micro-grafting onto rootstocks of trifoliate orange and acclimatization in a plastic greenhouse.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
Literature Cited

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