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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국실험동물학회 Laboratory Animal Research THE KOREAN JOURNAL OF LABORATORY ANIMAL SCIENCE Vol.20 No.3
- 발행연도
- 2004.9
- 수록면
- 229 - 233 (5page)
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초록· 키워드
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In the past, intracytoplasmic sperm injection (ICSI) studies have indicated significant variation in ICSI success rates among different species, including zebrafish. In this study, to evaluate the potential for fertilization by sperm injection into fish eggs, sperm from zebrafish (Danio rerio) were microinjected into egg cytoplasm. Four different combinations of injection conditions were conducted using activated or nonactivated gametes. From a total of 219 zebrafish eggs injected with sperm in all treatments, 25 (11.4%) developed to blastula, 9 (4.1%) developed to gastrula, 2 (1.0%) developed abnormally to larval stages, and as a result none developed normally. There seems to be something in the buffer that is not optimal, so injecting sperm into the eggs causes abnormal development in all embryos. The highest fertilization rate (blastodisc formation) was achieved by injection of activated spermatozoa into nonactivated eggs (16.1 %). This technique enables the generation of ubiquitously expressing transgenic zebrafish directly by microinjection. The method described here is likely to be applicable to other teleosts, such as medaka and salmon.
목차
Introduction
Materials and Methods
Results
Discussion
Acknowledgments
References
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