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Light intensity, a major determinant in creating infarct lesion in photothrombotic model, has never been systematically investigated. Authors varied light intensity to demonstrate diverse depth of lesions, correlating with motor deficit and its natural recovery pattern and thus tried to establish an individualized infarct model which can meet specific purpose of other stroke researches. Sprague-Dawley male rats were trained to perform single pellet reaching task (SPRT) for 2 weeks preoperatively. Photothrombotic infarcts were made on the sensorimotor cortex contralateral to preffered limb using rose-bengal dye (20 ㎎/㎏) and cold light. Seventy-five rats were divided depending on the light energy (W/㎠); sham control (n=15), 0.2 (n=15), 0.5 (n=15), 0.75 (n=15), and 1.0 (n=15). Each nine rats (total forty-five) were sampled one day after operation for imaging analysis of infarct lesions by MRI. SPRT were continued up to 2 weeks after operation to determine the recovery patterns. As the light intensity is increased, the depth of infarct lesion was deeper. The depth of infarct lesion is linearly correlated with the light intensity. Immediately after infarction, all the experimental groups showed sharp decline of SPRT scores and then showed slowely improved motor function up to 2 weeks. Final recoveries were statistically different among group sham, 0.2, 0.5, 0.75, and 1.0 W/㎠, It is concluded that light intensity can modulate the extent of infarct lesion, correlating with final recovery of motor behavior. Modulation of light intensity is contributing to choose the optimal ischemia model suited for specific purpose.

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UCI(KEPA) : I410-ECN-0101-2009-510-016363128