지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.3
- 수록면
- 59 - 68 (10page)
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초록· 키워드
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N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 ㎎/㎏ ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho's Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in G₁, G₂and G₃mice. One hundred thirty five G₁phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen G₃recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.
목차
INTRODUCTION
MATERIALDS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENT
REFERENCES
참고문헌 (24)
참고문헌 신청
Bode, V.C , 1984 , Ethylnitrosourea mutagenesis and the isolation of mutant alleles for specific genes located in the T region of mouse chromosome 17 , Genetics 108 : 457 ~ 470
Brinster, R.L , 1984 , Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors , Cell 37 : 367 ~ 379
Brown, S.D , 1999 , Mouse mutagenesissystematic studies of mammalian gene function , Hum. Mol. Genet 7 : 1627 ~ 1633
Davis, A.P , 1999 , Effective chemical mutagenesis in FVB, N mice requires low doses of ethylnitrosourea , Mamm Genome 10 : 308 ~ 310
Dominguez-Bendala, J , 2003 , Elevated expression of exogenous Rad51 leads to identical increases in gene-targeting frequency in murine embryonic stem (ES) cells with both functional and dysfunctional p53 genes , Exp. Cell Res 286 : 298 ~ 307
Brinster, R.L , 1984 , Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors , Cell 37 : 367 ~ 379
Brown, S.D , 1999 , Mouse mutagenesissystematic studies of mammalian gene function , Hum. Mol. Genet 7 : 1627 ~ 1633
Davis, A.P , 1999 , Effective chemical mutagenesis in FVB, N mice requires low doses of ethylnitrosourea , Mamm Genome 10 : 308 ~ 310
Dominguez-Bendala, J , 2003 , Elevated expression of exogenous Rad51 leads to identical increases in gene-targeting frequency in murine embryonic stem (ES) cells with both functional and dysfunctional p53 genes , Exp. Cell Res 286 : 298 ~ 307
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UCI(KEPA) : I410-ECN-0101-2009-513-016438012