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The change of microbial flora in intestine and respiratory organs make some effects on animal health, which can cause some aberrations to experiment data. Therefore, it is an important factor to maintain the homogeneity of the bacterial composition of each organ. In this study, the bacterial distribution of respiratory organs and intestine were analyzed from 247 mice and 200 rats by analyzing biochemical properties and 16S rDNA sequencing. The total of 1,075 isolates were classified to 35 species of 17 genera. In case of rats, intestinal microbial flora were composed of 5 major bacteria including E. coli, P. mirabilis, M. morganii, C. freundii, and P. vulgaris, and 5 bacteria such as E. coli, P. mirabilis, S. aureus, S. cohnii, and M. morganii also mainly consisted of the microbial flora of respiratory organs. In case of mice, intestinal microbial flora were composed of 4 major bacteria including E. coli, P. mirabilis, A. lwoffi, and L. adecarboxylata, and 5 bacteria such as E. coli, B. sphaericus, S. auricularis, A. lwoffi, and L. adecarboxylata also mainly consisted of microbial flora of respiratory organs. This study suggested that B. sphaericus, G. morbillorum, S. paucimobilis, Staphylococcus spp. were specific in respiratory organs, and that M. morganii and Citrobacter spp. were almost specific in intestine. This study also can give some basic information to understand the relationship between animal care environment and microbial composition of animal organs.

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UCI(KEPA) : I410-ECN-0101-2009-510-014753493