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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.49 No.5
발행연도
2008.10
수록면
347 - 351 (5page)

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A number of transformation methods have been developed to transfer important genes into plant cells. Selectable marker genes are needed in all plant transformation approaches. Since most selectable marker genes, including those conferring resistance to clinically not important antibiotics, raise significant safety concerns to public, their removal should help eliminate public concerns over the safety of transgenic plants. Purpose of this study was to develop of selective marker gene-free transgenic plants using Cre-lox system. Tobacco plants carrying the nptⅡ flanked by two lox sites and transgenic tobacco plants harboring the cre gene were developed. Each lox containing plant was also retransformed with a Cre expressing plasmid. The recombination event could be detected since it places the CaMV 35S promoter of the nptⅡ gene adjacent to a promoterless gusA gene. As a result the gusA gene was activated and its expression could be visualized. Cre-lox system from bacteriophage P1 was tested to investigate its ability to precise excision of stably integrated marker genes from chromosomes in transgenic tobacco plants.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgment
Literature Cited

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