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초록·키워드
Effects of Korean mistletoe extracts (KM-110), Viscum album coloratum on the specific and non-specific immune responses of Japanese eel Anguilla japonica were examined. The optimal concentration not showing toxicity of KM-110 was determined to 30-40 ㎍/ml in vitro and 100 ㎍/100 g of fish in vivo. Even 1000 ㎍ of KM-110/100 g of fish did not show any clinical problem in fish though the levels of toxic parameters were slightly increased. In terms of antibody production, KM-110 significantly elicited more antibody production than FCA or β-glucan. β-glucan plus KM-110 group synergistically enhanced antibody production. There was no significant difference between KM-110 and KM-110 plus β-glucan group. The ROI production by head kidney (HK) leu-cocytes of eel injected with 500 or 1000㎍ KM-110 was significantly (P＜0.05) enhanced than the control and FCA-treated group. Maximum increase in the NBT reduction value was observed in 1000 ㎍ KM-110 group but no significant difference was found between 500 and 1000 ㎍ KM group. The level of serum lysozyme activity was significantly (P＜0.05) higher in the 500 and 1000 ㎍ KM-110-or FCA-treated group than in the control and 200 ㎍ KM-110 group. The phagocytic activities of HK leucocytes isolated from eel injected with 500 and 1000 ㎍ KM-110 were significantly (P＜0.05) higher than 200 ㎍ KM-110 and PBS-injected control group. Korean mistletoe appeared to be a good activator of the specific and non-specific immune responses of Japanese eel.
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