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자료유형
학술저널
저자정보
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한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.51 No.1
발행연도
2010.2
수록면
45 - 50 (6page)

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In order to determine the medium conditions of liquid stationary culture for the maximum bulblet enlargement, in vitro bulblets induced from bulb scales using solidified medium of six Korean native lilies (Lilium dauricum, L. hansonii, L. maximowitzii, L. amabile, L. concolor var. parthneion, and L. tsingtauense) were cultured in various strengths (1/8, 1/4, 1/2, and 1 fold) and volumes (10, 30, 50, 70, and 90 mL in a 350 mL container) of MS medium, and sucrose concentrations (30, 45, 60, and 90 gㆍL?¹) for 16 weeks. Solidified medium culture as the control was conducted on 30 mL of the basal culture medium (MS salt, 60 gㆍL?¹ sucrose, and pH 5.7) containing 7 gㆍL?¹ agar. Compared with conventional solid culture, bulblet enlargement was promoted by liquid stationary culture in 114% to 215% depending on species. The best concentration of MS medium for bulblet enlargement was the full strength (one fold) in all species tested. Fresh weight of bulblets was increased by gradual increasing of sucrose concentration from 30 to 90 gㆍL?¹, which 90 gㆍL?¹ sucrose was best in L. hansonii, L. concolor var. parthneion, L. tsingtauense, and L. amabile, and 60 gㆍL?¹ was the most effective in bulblet enlargement of L. dauricum and L. maximowitzii. The adequate volume of medium was 30 mL in L. amabile, L. dauricum, L. hansonii, and L. maximowitzii, and 50 mL in L. concolor and L. tsingtauense in a 350 mL container.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2010-525-002268265