지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 141 - 145 (5page)
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초록· 키워드
오류제보하기
Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Many species of mycoplasmas are important pathogens
that cause respiratory infection in laboratory animals and that are known to affect experimental results obtained with contaminated animals. The aim of the present study was to develop a sensitive and specific assay for the detection of mycoplasma species. To this end, we developed a polymerase chain reaction and dot blot hybridization assay (PCR/DBH) for detecting mycoplasma DNA and evaluated it for its sensitivity and specificity. Mycoplasma consensus primer pairs were used for the amplification of target DNA. When PCR product was visually detected, the limit of detection of the PCR test was 10² pg of mycoplasma purified DNA. For DBH, the amplified DNA was labeled by incorporation of digoxigenin (DIG). This DIG-labeled probe was capable of detecting 10⁴ pg of purified mycoplasma DNA by DBH. PCR/DBH was more sensitive than PCR or DBH alone and was also very specific. Our PCR/DBH assay can be applied efficiently to confirm the presence of mycoplasma species on clinical samples and to differentiate between mycoplasma species infection and other bacterial infections.
that cause respiratory infection in laboratory animals and that are known to affect experimental results obtained with contaminated animals. The aim of the present study was to develop a sensitive and specific assay for the detection of mycoplasma species. To this end, we developed a polymerase chain reaction and dot blot hybridization assay (PCR/DBH) for detecting mycoplasma DNA and evaluated it for its sensitivity and specificity. Mycoplasma consensus primer pairs were used for the amplification of target DNA. When PCR product was visually detected, the limit of detection of the PCR test was 10² pg of mycoplasma purified DNA. For DBH, the amplified DNA was labeled by incorporation of digoxigenin (DIG). This DIG-labeled probe was capable of detecting 10⁴ pg of purified mycoplasma DNA by DBH. PCR/DBH was more sensitive than PCR or DBH alone and was also very specific. Our PCR/DBH assay can be applied efficiently to confirm the presence of mycoplasma species on clinical samples and to differentiate between mycoplasma species infection and other bacterial infections.
목차
Material and Methods
Results
Discussion
Acknowledgments
References
참고문헌 (30)
참고문헌 신청
McAuliffe L / 2003 / Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting / J Clin Microbiol 41(10):4844~4847
Delgado MO / 2001 / Immunoblot profiles of sera from laboratory rats naturally infected with Mycoplasma pulmonis and technicians exposed to infected animal facilities / Braz J Microbiol 32(4):301~304
Seung-Hee Kim / 2005 / Genus-Specific Polymerase Chain Reaction to Detect Mycoplasma Species / Laboratory Animal Research 21(3):189~192
Muthomi EK / 1983 / Passive haemagglutination and complement fixation as diagnostic tests for contagious caprine pleuropneumonia caused by the F-38 strain of mycoplasma / Res Vet Sci 35(1):1~4
Ball HJ / 1998 / Diagnostic application of monoclonal antibody (MAb)-based sandwich ELISAs / Methods Mol Biol 104:127~132
Delgado MO / 2001 / Immunoblot profiles of sera from laboratory rats naturally infected with Mycoplasma pulmonis and technicians exposed to infected animal facilities / Braz J Microbiol 32(4):301~304
Seung-Hee Kim / 2005 / Genus-Specific Polymerase Chain Reaction to Detect Mycoplasma Species / Laboratory Animal Research 21(3):189~192
Muthomi EK / 1983 / Passive haemagglutination and complement fixation as diagnostic tests for contagious caprine pleuropneumonia caused by the F-38 strain of mycoplasma / Res Vet Sci 35(1):1~4
Ball HJ / 1998 / Diagnostic application of monoclonal antibody (MAb)-based sandwich ELISAs / Methods Mol Biol 104:127~132
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UCI(KEPA) : I410-ECN-0101-2013-510-000557359