지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 135 - 140 (6page)
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초록· 키워드
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We compared three EDTA-based phenotypic screening methods for detecting IMP-1 and VIM-2 type metallo-β-lactamase (MBL)-producing isolates of Acinetobacter and Pseudomonas spp., EDTA-double disk synergy test (EDTADDST), Etest MBL, and imipenem (IPM)-EDTA disk test. A total of 183 isolates (65 Acinetobacter spp. and 118 Pseudomonas spp. showing IPM resistance), confirmed to MBL genes by PCR, were used. The criteria for MBL production were (ⅰ) presence of a synergistic zone between IPM and EDTA disks in EDTA-DDST, (ⅱ) reduction of IPM minimal inhibitory concentration by ≥ 3 twofold dilutions in the presence of EDTA in the Etest MBL, and (ⅲ) ≥ 7 ㎜ increase in the inhibition zone around the IPM plus EDTA disks compared with a sole IPM disk in the IPM-EDTA disk test. In this study using 87 MBL-producing and 96 MBL-nonproducing isolates, the sensitivities/specificities of EDTA-DDST, Etest MBL and IPM-EDTA disk tests were 94.3/78.1%, 89.7/91.7%, and 97.7/95.8%, respectively. When the threshold for the increase of the inhibition zone around the IPM plus EDTA disk over a sole IPM disk was altered to ≥ 5 ㎜ and ≥ 8 ㎜ for Acinetobacter spp. and Pseudomonas spp., respectively, the sensitivity and specificity of the test were 98.9% and 96.9%, respectively. Of the three EDTA-based phenotypic tests, the IMP-EDTA disk test was superior for detection of MBL-producing isolates.
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서론
재료 및 방법
결과 및 고찰
REFERENCES
참고문헌 (23)
참고문헌 신청
Arakawa Y / 2000 / Convenient test for screening metallo-β-lactamase-producing gram-negative bacteria by thiol compounds / J Clin Microbiol 38:40~43
Bush K / 1995 / A functional classification scheme for β-lactamases and its correlation with molecular structure / Antimicrob Agents Chemother 39:1211~1233
Castanheira M / 2004 / Molecular characterization of a β-lactamase gene, blaGIM-1, encoding a new subclass of metallo-β-lactamase / Antimicrob Agents Chemother 48:4654~4661
Chu YW / 2005 / EDTA susceptibility leading to false detection of metallo-β-lactamase in Pseudomonas aeruginosa by Etest and an imipenem-EDTA disk method / Intern J Antimicrob Agents 26:338~341
Clinical and Laboratory Standards Institute / 2006 / Performance standards for antimicrobial susceptibility testing. Sixteenth informational supplement / Wayne, Pa
Bush K / 1995 / A functional classification scheme for β-lactamases and its correlation with molecular structure / Antimicrob Agents Chemother 39:1211~1233
Castanheira M / 2004 / Molecular characterization of a β-lactamase gene, blaGIM-1, encoding a new subclass of metallo-β-lactamase / Antimicrob Agents Chemother 48:4654~4661
Chu YW / 2005 / EDTA susceptibility leading to false detection of metallo-β-lactamase in Pseudomonas aeruginosa by Etest and an imipenem-EDTA disk method / Intern J Antimicrob Agents 26:338~341
Clinical and Laboratory Standards Institute / 2006 / Performance standards for antimicrobial susceptibility testing. Sixteenth informational supplement / Wayne, Pa
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UCI(KEPA) : I410-ECN-0101-2013-510-000412762