지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 151 - 155 (5page)
이용수
초록· 키워드
오류제보하기
Short tandem repeats (STRs) loci are the genetic markers used for forensic human identity test. With multiplex polymerase chain reaction (PCR) assays, STRs are examined and measured PCR product length relative to sequenced allelic ladders. In the repeat region and the flanking region of the commonly-used STR may have DNA sequence variation. A mismatch due to sequence variation in the DNA template may cause allele drop-out (i.e., a “null” or “silent” allele) when it falls within PCR primer binding sites. The STR markers were co-amplified in a single reaction by using commercial PowerPlex<SUP>®</SUP> 16 system and AmpFlSTR<SUP>®</SUP> Identifiler<SUP>®</SUP> PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI PRISM<SUP>®</SUP> 3100 Genetic Analyzer with capillary electrophoresis. The GeneMapperTM ID software were used for size calling and analysis of STR profiles. Here, this study described a forensic human identity test in which allelic drop-out occurred in the STR system D18S51. During the course of human identity test, two samples with a homozygous (16, 16 and 21, 21) null genotype at D18S51 locus were discovered using the PowerPlex<SUP>®</SUP> 16 system. The loss of alleles was confirmed when the samples were amplified using AmpFlSTR<SUP>®</SUP> Identifiler<SUP>®</SUP> PCR amplification kit and resulted in a heterozygous (16, 20 and 20, 21) genotype at this locus each other. This discrepancy results suggest that appropriate measures should be taken for database comparisons and that null allele should be further investigated by sequence analysis and be reported to the forensic community.
목차
서론
재료 및 방법
결과
고찰
감사의 글
REFERENCES
참고문헌 (19)
참고문헌 신청
Amorin A / 2004 / Progress in forensic genetics: Genotyping inconsistencies and null alleles using AmpFlSTR® Identifiler® and PowerPlex® 16 kits. Vol 10 / Experta Medica, Elsevier. Amsterdam, Nederland :176~178
Boutrand L / 2001 / Variations in primer sequences are the origin of allele drop-out at loci D13S317 and CD4 / Int J Legal Med 114:295~297
Budowle B / 1999 / Population data on the thirteen CODIS core short tandem repeat loci in African Americans, US Caucasians, Hispanics, Bahamians, Jamaicans and Trinidadians / J Forensic Sci 44:1277~1286
Budowle B / 2001 / STR primer concordance study / Forensic Sci Int 124:47~54
Clayton T / 2004 / Primer binding site mutation affecting the typing of STR loci contained within the AmpFlSTR® SGMTM Plus kit / Forensic Sci Int 139:255~259
Boutrand L / 2001 / Variations in primer sequences are the origin of allele drop-out at loci D13S317 and CD4 / Int J Legal Med 114:295~297
Budowle B / 1999 / Population data on the thirteen CODIS core short tandem repeat loci in African Americans, US Caucasians, Hispanics, Bahamians, Jamaicans and Trinidadians / J Forensic Sci 44:1277~1286
Budowle B / 2001 / STR primer concordance study / Forensic Sci Int 124:47~54
Clayton T / 2004 / Primer binding site mutation affecting the typing of STR loci contained within the AmpFlSTR® SGMTM Plus kit / Forensic Sci Int 139:255~259
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UCI(KEPA) : I410-ECN-0101-2013-510-000412782