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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 209 - 218 (10page)
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초록· 키워드
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Ginseng has been used as a general tonic agent to invigorate the human body as an adaptogenic agent. In a previous report, we have shown that ginseng contains a novel glycolipoprotein called gintonin. The main function of gintonin is to transiently enhance intracellular free Ca²? [Ca²?]i levels in animal cells. The previous method for gintonin isolation included multiple steps using organic solvents. In the present report, we developed a simple method for the preparation of crude gintonin from ginseng root as well as stem and leaf, which produced a higher yield of gintonin than the previous one. The yield of gintonin was 0.20%, 0.29%, and 0.81% from ginseng root, stem, and leaf, respectively. The apparent molecular weight of gintonin isolated from stem and leaf through sodium dodecyl sulfate polyacrylamide gel electrophoresis was almost same as that from root but the compositions of amino acids, carbohydrates or lipids differed slightly between them. We also examined the effects of crude gintonin from ginseng root, stem, and leaf on endogenous Ca²?-activated Cl- channel (CaCC) activity of Xenopus oocytes through mobilization of [Ca²?] i. We found that the order of potency for the activation of CaCC was ginseng root ＞ stem ＞ leaf. The ED?? was 1.4±1.4, 4.5±5.9, and 3.9±1.1 ㎎/mL for root, stem and leaf, respectively. In the present study, we demonstrated for the first time that in addition to ginseng root, ginseng stem and leaf also contain gintonin. Gintonin can be prepared from a simple method with higher yield of gintonin from ginseng root, stem, and leaf. Finally, these results demonstrate the possibility that ginseng stem and leaf could also be utilized for ginstonin preparation after a simple procedure, rather than being discarded.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (10)
참고문헌 신청
1. [학술지(정기간행물)] - Nah SY / 1997 / Ginseng: recent advances and trends / Korean J Ginseng Sci 21 : 1 ~ 12
2. [학술지(정기간행물)] - / 2001 / G alpha(q/11) coupled to mammalian phospholipase C beta 3-like enzyme mediates the ginsenoside effect on Ca2+-activated Cl- current in the Xenopus oocyte / JOURNAL OF BIOLOGICAL CHEMISTRY 276 : 48797 ~ 48802
3. [학술지(정기간행물)] - Lee JH / 2004 / Prevention of ginsenoside-induced desensitization of Ca2+-activated Cl- current by microinjection of inositol hexakisphosphate in Xenopus laevis oocytes - Involvement of GRK2 AND beta-arrestin I / JOURNAL OF BIOLOGICAL CHEMISTRY 279 (11) : 9912 ~ 9921
4. [학술지(정기간행물)] - Jeong SM / 2004 / Ginseng saponins induce store-operated calcium entry in Xenopus oocytes / British Journal of Pharmacology 142 (3) : 585 ~ 593
6. [학술지(정기간행물)] - Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 248 ~ 254
2. [학술지(정기간행물)] - / 2001 / G alpha(q/11) coupled to mammalian phospholipase C beta 3-like enzyme mediates the ginsenoside effect on Ca2+-activated Cl- current in the Xenopus oocyte / JOURNAL OF BIOLOGICAL CHEMISTRY 276 : 48797 ~ 48802
3. [학술지(정기간행물)] - Lee JH / 2004 / Prevention of ginsenoside-induced desensitization of Ca2+-activated Cl- current by microinjection of inositol hexakisphosphate in Xenopus laevis oocytes - Involvement of GRK2 AND beta-arrestin I / JOURNAL OF BIOLOGICAL CHEMISTRY 279 (11) : 9912 ~ 9921
4. [학술지(정기간행물)] - Jeong SM / 2004 / Ginseng saponins induce store-operated calcium entry in Xenopus oocytes / British Journal of Pharmacology 142 (3) : 585 ~ 593
6. [학술지(정기간행물)] - Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 248 ~ 254
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