지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 205 - 212 (8page)
이용수
초록· 키워드
오류제보하기
Embryonic stem cells (ESCs) are an emerging source for cell-based therapies aimed at repairing damaged organ tissues; however, the efficiency of directed differentiation is low and refinement of differentiation protocols is hampered by incomplete understanding of the mechanisms involved in this process. To find new compounds which can improve the efficiency of directed differentiation of ESCs to cardiomyocytes, we screened several thousand chemical compounds and identified a promising group. All of the compounds found have a common structure of 1H-pyrrole,2,2'-(phenylmethylene)bis. Here we report the potential mechanism of action for 31002 which showed the strongest activity among the compounds selected. In the presence of 31002, 15 times more cardiomyocytes differentiated from ESCs, i.e., 3.5% to 52% of total differentiated cells. Moreover, the cardiomyocytes showed functional characteristics including rhythmic beating and marker gene expression. 31002 inhibited the down-regulation of genes related to the three germ layers in the late stage of ESCs differentiation, implying that 31002 supports a continuous fate commitment of undifferentiated ESCs to the cardiac lineage by prolonging the three germ layer stages. Therefore, compounds in this group, including 31002, might be useful as directed cardiomyogenic differentiation-inducers to produce cells for use in cell therapy aimed at restoring damaged heart tissue.
목차
Materials and Methods
Results
Acknowledgments
References
참고문헌 (27)
참고문헌 신청
Cohn JN / 1997 / Report of the National Heart, Lung, and Blood Institute Special Emphasis Panel on Heart Failure Research / Circulation 95(4):766~770
Puceat M / 2008 / Human embryonic stem cell: is it a realistic cell source for regenerative therapy of heart failure / Pathol Biol (Paris) 56(2):47~49
Pasumarthi KB / 2002 / Cardiomyocyte cell cycle regulation / Circ Res 90(10):1044~1054
Kusumoto FM / 1996 / Cardiac pacing / N Engl J Med 334(2):89~97
Kehat I / 2001 / Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes / J Clin Invest 108(3):407~414
Puceat M / 2008 / Human embryonic stem cell: is it a realistic cell source for regenerative therapy of heart failure / Pathol Biol (Paris) 56(2):47~49
Pasumarthi KB / 2002 / Cardiomyocyte cell cycle regulation / Circ Res 90(10):1044~1054
Kusumoto FM / 1996 / Cardiac pacing / N Engl J Med 334(2):89~97
Kehat I / 2001 / Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes / J Clin Invest 108(3):407~414
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2013-510-000764430