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논문 기본 정보

자료유형
학술저널
저자정보
이소연 (성신여자대학교) 위해리 (성신여자대학교) 이명숙 (성신여자대학교)
저널정보
한국영양학회 한국영양학회지 한국영양학회지 제44권 제6호
발행연도
2011.12
수록면
488 - 497 (10page)

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Oxygen is necessary to sustain life, yet cellular oxygen metabolism creates destructive elements called free radicals. Free radicals are chemically unbalanced and carrying free electrons that can damage molecules, potentially damaging the cell itself. For this reason, many antioxidant products, including supplements and functional foods, are being developed. In particular, natural products are rich sources of pharmacologically active compounds. The purpose of this study was to investigate the antioxidant effects of target biomaterials in Korean traditional spices such as diallyl sulfide (DAS), capsaicin (CAP), and gingerol (GGR), and to investigate the response of the antioxidant defense system to oxidative stress by hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) compared to sulforaphane (SFN) in HepG2 cells. After the analysis of the cell viability using Cell Counting kit-8 (CCK-8) assay, we determined that the optimum levels were 200 μM DAS, 25 μM CAP, 50 μM GGR, and 12.5 μM SFN. Antioxidant enzymes were measured and protein expression was detected by Western blotting. All treatments showed a significant decrease in antioxidant enzyme activity such as superoxide dismutase, catalse, and glutathione peroxidase in HepG2 cells. Additionally, DAS, CAP, GGR and SFN increased the antioxidant system-related transcription factor Nrf2 which was found to be regulated by the activation of MAPK-JNK in this study. In conclusion, these results indicate the protective effects of DAS CAP, GGR, and SFN against H<SUB>2</SUB>O<SUB>2</SUB>-induced oxidative stress. (Korean J Nutr 2011; 44(6): 488 ~ 497)

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UCI(KEPA) : I410-ECN-0101-2013-594-001198923