지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 504 - 513 (10page)
이용수
초록· 키워드
오류제보하기
In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP II 0, and PGP 130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as "ATA", "GCC", "GTA", "GCA", and" ACC", respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (34)
참고문헌 신청
1. [학술지(정기간행물)] - Park CK / 2003 / The chemical components of Korean ginseng / Food Ind Nutr 8 : 10 ~ 23
2. [학술지(정기간행물)] - Wang LC / 2000 / Effect of ginseng saponins on cold tolerance in young and elderly rats / Planta Med 66 : 144 ~ 147
3. [학술지(정기간행물)] - Shin HR / 2000 / The cancer-preventive potential of Panax ginseng: a review of human and experimental evidence / Cancer Causes Control 11 : 565 ~ 576
4. [학술지(정기간행물)] - Yun TK / 2001 / Anticarcinogenic effect of Panax ginseng C.A. Meyer and identification of active compounds / J Korean Med Sci 16 : S6 ~ S18
5. [학술지(정기간행물)] - Kim SH / 2003 / Effects of Panax ginseng extract on lipid metabolism in humans / Pharmacol Res 48 : 511 ~ 513
2. [학술지(정기간행물)] - Wang LC / 2000 / Effect of ginseng saponins on cold tolerance in young and elderly rats / Planta Med 66 : 144 ~ 147
3. [학술지(정기간행물)] - Shin HR / 2000 / The cancer-preventive potential of Panax ginseng: a review of human and experimental evidence / Cancer Causes Control 11 : 565 ~ 576
4. [학술지(정기간행물)] - Yun TK / 2001 / Anticarcinogenic effect of Panax ginseng C.A. Meyer and identification of active compounds / J Korean Med Sci 16 : S6 ~ S18
5. [학술지(정기간행물)] - Kim SH / 2003 / Effects of Panax ginseng extract on lipid metabolism in humans / Pharmacol Res 48 : 511 ~ 513
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UCI(KEPA) : I410-ECN-0101-2013-524-001212695