지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 47 - 54 (8page)
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초록· 키워드
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Korean red ginseng (KRG) has been used worldwide as a traditional medicine for the treatment of various reproductive diseases. Gonadotropin releasing hormone (GnRH) neurons are the fundamental regulators of pulsatile release of gonadotropin required for fertility. In this study, an extract of KRG (KRGE) was applied to GnRH neurons to identify the receptors activated by KRGE. The brain slice patch clamp technique in whole cell and perforated patch was used to clarify the effect of KRGE on the membrane currents and membrane potentials of GnRH neurons. Application of KRGE (3 ㎍/μL) under whole cell patch induced remarkable inward currents (56.17±7.45 pA, n=25) and depolarization (12.91±3.80 ㎷, n=4) in GnRH neurons under high Cl? pipette solution condition. These inward currents were not only reproducible, but also concentration dependent. In addition, inward currents and depolarization induced by KRGE persisted in the presence of the voltage gated Na? channel blocker tetrodotoxin (TTX), suggesting that the responses by KRGE were postsynaptic events. Application of KRGE under the gramicidin perforated patch induced depolarization in the presence of TTX suggesting its physiological signifi cance on GnRH response. Further, the KRGEinduced inward currents were partially blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; non-NMDA glutamate receptor antagonist, 10 μM) or picrotoxin (PIC; GABA<SUB>A</SUB> receptor antagonist, 50 μM), and almost blocked by PIC and CNQX mixture. Taken together, these results suggest that KRGE contains ingredients with possible GABA and non-NMDA glutamate receptor mimetic activity, and may play an important role in the endocrine function of reproductive physiology, via activation of GABA<SUB>A</SUB> and non-NMDA glutamate receptors in GnRH neurons.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (38)
참고문헌 신청
1. [학술지(정기간행물)] - Sisk CL / 2004 / The neural basis of puberty and adolescence / Nat Neurosci 7 : 1040 ~ 1047
2. [학술지(정기간행물)] - Belchetz PE / 1978 / Hypophysial responses to continuous and intermittent delivery of hypopthalamic gonadotropin-releasing hormone / Science 202 : 631 ~ 633
3. [학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology : multiple constituents and multiple actions / Biochem Pharmacol 58 : 1685 ~ 1693
4. [학술지(정기간행물)] - Tachikawa E / 1999 / Effects of ginseng saponins on responses induced by various receptor stimuli / Eur J Pharmacol 369 : 23 ~ 32
5. [학술지(정기간행물)] - Yun TK / 2001 / Brief introduction of Panax ginseng C.A. Meyer / J Korean Med Sci 16 (Suppl) : S3 ~ S5
2. [학술지(정기간행물)] - Belchetz PE / 1978 / Hypophysial responses to continuous and intermittent delivery of hypopthalamic gonadotropin-releasing hormone / Science 202 : 631 ~ 633
3. [학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology : multiple constituents and multiple actions / Biochem Pharmacol 58 : 1685 ~ 1693
4. [학술지(정기간행물)] - Tachikawa E / 1999 / Effects of ginseng saponins on responses induced by various receptor stimuli / Eur J Pharmacol 369 : 23 ~ 32
5. [학술지(정기간행물)] - Yun TK / 2001 / Brief introduction of Panax ginseng C.A. Meyer / J Korean Med Sci 16 (Suppl) : S3 ~ S5
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UCI(KEPA) : I410-ECN-0101-2013-524-001261138