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한국식품과학회 한국식품과학회지 한국식품과학회지 제15권 제2호
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    초록·키워드

    Direct conversion of starchy materials to single cell protein of Sporobolomyces holsaticus FRI Y-5 was investigated. Effect of yeast extract concentration on its cell growth showed that it could utilize more of starch in the medium containing 2.5 g/l of yeast extract. In case of jar fermentor culture, the specific growth rate and cell yield of Sp. holsaticus on soluble starch were calculated to be 0.14 hr⁻¹ and 0.425, respectively and its maximum cell concentration was 13.4 g/l. After 80 hr of incubation time, 45.96% of starch was consumed and 45.1% of relative blue value was decreased. Reducing sugars in the starch medium seemed to increase from 4.06 g/l to 6.08 g/l and then to decrease. During fermentor culture, pH of medium was almost not changed in the range of pH 7.0±0.5. The optimal temperature and pH of Sp. holsaticus amylase activity were 40 °C and pH 7.5, respectively. It was shown from the effect of Tapioca starch concentration on the cell growth that the optimal concentration of Tapioca starch for Sp. holsaticus was lower than that of soluble starch. FRI Y-5 cells settled much slower than Sp. holsaticus IFO 1032 cells and the viscosity vs cell concentration relationship was related to be linear.

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      UCI(KEPA) : I410-ECN-0101-2013-573-001523809