지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.3
- 수록면
- 39 - 49 (11page)
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초록· 키워드
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We investigated the effect of zinc on the formation of colonic aberrant crypt foci induced by azoxymethane (AOM) followed by dextran sodium sulfate (DSS) in mice with high iron diet (HFe; 450 ppm iron). Sixweek old ICR mice were fed on high iron diets with combination of three different levels of zinc in diets, low-zinc (LZn; 0.01 ppm), medium-zinc (MZn; 0.1 ppm), and high-zinc (HZn; 1 ppm) for 12 weeks. Animals were received weekly intraperitoneal injections of AOM (10 mg/kg B.W. in saline) for 3 weeks followed by 2% DSS (molecular weight 36,000~50,000) in the drinking water for a week. To confirm the iron storage in the body, the hepatic iron concentration has been determine chemically and compared with histological assessment visualized by Prussian blue reaction. Aberrant crypt (AC) and aberrant crypt foci (ACF) were analyzed in the colonic mucosa of mouse fed high dietary iron. Superoxide dismutase (SOD) activity and thiobarbituric acid-reactive substances (TBARS) level were also investigated. Apoptosis in the preneoplastic lesion was determined by terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL). In addition, immunohistochemistry of β-catenin was also performed on the mucous membrane of colon. The number of large ACF (≥ 4 AC/ACF), which possess greater tumorigenic potential, was significantly lower in MZn and HZn groups compared with LZn group. Cytosolic SOD activity in the liver was significantly higher in HZn group compared with LZn group. Hepatic MDA level was decreased significantly in HZn group compared with MZn and LZn groups. Apoptotic index was significantly higher in HZn group. Taken together, these findings indicate that dietary zinc might exert a protective effect against colonic preneoplastic lesion induced by AOM/DSS in ICR mice with high iron status, and suggest that dietary supplement of zinc might play a role in suppressing colon carcinogenesis in mice.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (39)
참고문헌 신청
Bartsch, H. / 2002 / Potential role of lipid peroxidation derived DNA damage in human colon carcinogenesis: studies on exocyclic base adducts as stable oxidative stress markers / Cancer Detect. Prev 26:308~312
Bettger, W. J. / 1980 / Interaction of zinc and vitamin E in the chick / Proc. Soc. Exp. Biol. Med 163:432~436
Bird, R. P. / 1998 / Aberrant crypt foci system to study cancer preventive agents in the colon. In Tumor Marker Protocols / Humana Press :465~474
Bird, R. P. / 1987 / Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: preliminary findings / Cancer Lett 37:147~151
Chang, W. W. / 1984 / Histogenesis of colon cancer in experimental animals / Scand. J. Gastroenterol. Suppl 104:27~43
Bettger, W. J. / 1980 / Interaction of zinc and vitamin E in the chick / Proc. Soc. Exp. Biol. Med 163:432~436
Bird, R. P. / 1998 / Aberrant crypt foci system to study cancer preventive agents in the colon. In Tumor Marker Protocols / Humana Press :465~474
Bird, R. P. / 1987 / Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: preliminary findings / Cancer Lett 37:147~151
Chang, W. W. / 1984 / Histogenesis of colon cancer in experimental animals / Scand. J. Gastroenterol. Suppl 104:27~43
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UCI(KEPA) : I410-ECN-0101-2013-513-001730002