지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.4
- 수록면
- 1,075 - 1,083 (9page)
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Purpose : This study is to investigate the effect of acute endurance exercise and recovery on expression Autophagy factor Beclin1 and LC3 in rat skeletal muscle
Method : The male Sprague-Dawley rats(n=65) were randomly placed in one of three groups(40% low intensity, 60% the middle intensity, 80% high intensity). The Rat in the exercised groups ran the treadmill running for the intensity 60min 7~26M/min with degree slope 10°. Soleus and Extensor digitorum longus muscle exercised 30,60min and during recovery 6, 12, 24h. The western blotting analysis performed for the expression of Beclin1 and LC3 protein.
Results : The expression of Beclin1 in Soleus middle and high intensity than low intensity. And the middle intensity 12h, high intensity 6h significantly increased than other. The expression of Beclin1 in Extensor digitorum longus gradually increased in follow during recovery . The expression of LC3-Ⅰ in soleus during acute exercise gradually decreased in intensity, however LC-Ⅱ middle intensity during exercise 30min significantly increased the other group. The expression of LC3-Ⅰ in soleus during recovery different of group and intensity and LC3Ⅱ gradually increased time. The expression of LC3-Ⅰ in Extensor digitorum longus all group increased in during exercise 30min, and LC3-Ⅱ displayed similar type of middle and high intensity group. The expression of LC3-Ⅰ in Extensor digitorum longus during recovery gradually increased in follow during time, and LC3-Ⅱ significantly increased high intensity recovery 12h.
Conclusion : This study can be concluded that acute exercise has positive effects on autophagy factor Beclin1 and LC3. this factor displayed different skeletal muscle type and intensity.
Method : The male Sprague-Dawley rats(n=65) were randomly placed in one of three groups(40% low intensity, 60% the middle intensity, 80% high intensity). The Rat in the exercised groups ran the treadmill running for the intensity 60min 7~26M/min with degree slope 10°. Soleus and Extensor digitorum longus muscle exercised 30,60min and during recovery 6, 12, 24h. The western blotting analysis performed for the expression of Beclin1 and LC3 protein.
Results : The expression of Beclin1 in Soleus middle and high intensity than low intensity. And the middle intensity 12h, high intensity 6h significantly increased than other. The expression of Beclin1 in Extensor digitorum longus gradually increased in follow during recovery . The expression of LC3-Ⅰ in soleus during acute exercise gradually decreased in intensity, however LC-Ⅱ middle intensity during exercise 30min significantly increased the other group. The expression of LC3-Ⅰ in soleus during recovery different of group and intensity and LC3Ⅱ gradually increased time. The expression of LC3-Ⅰ in Extensor digitorum longus all group increased in during exercise 30min, and LC3-Ⅱ displayed similar type of middle and high intensity group. The expression of LC3-Ⅰ in Extensor digitorum longus during recovery gradually increased in follow during time, and LC3-Ⅱ significantly increased high intensity recovery 12h.
Conclusion : This study can be concluded that acute exercise has positive effects on autophagy factor Beclin1 and LC3. this factor displayed different skeletal muscle type and intensity.
목차
Abstract
Ⅰ. 서론
Ⅱ. 연구방법
Ⅲ. 결과
Ⅳ. 논의
Ⅴ. 결론 및 제언
참고문헌
참고문헌 (17)
참고문헌 신청
2. [학술지(정기간행물)] - Barth S / 2010 / Autophagy: assays and artifacts / J Pathol 221 (2) : 117 ~ 124
3. [학술지(정기간행물)] - Cao Y / 2007 / Physiological functions of Atg6/Beclin 1: a unique autophagy-related protein / Cell Res 17 (10) : 839 ~ 849
4. [학술지(정기간행물)] - Chen Y / 2010 / Methods for detecting autophagy and determining autophagy-induced cell death / Can J Physiol Pharmacol 88 (3) : 285 ~ 295
5. [학술지(정기간행물)] - Eskelinen EL / 2009 / Autophagy: a lysosomal degradation pathway with a central role in health and disease / Biochim Biophys Acta 1793 (4) : 664 ~ 673
6. [학술지(정기간행물)] - Kabeya Y / 2000 / LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing / EMBO J 19 (21) : 5720 ~ 5728
3. [학술지(정기간행물)] - Cao Y / 2007 / Physiological functions of Atg6/Beclin 1: a unique autophagy-related protein / Cell Res 17 (10) : 839 ~ 849
4. [학술지(정기간행물)] - Chen Y / 2010 / Methods for detecting autophagy and determining autophagy-induced cell death / Can J Physiol Pharmacol 88 (3) : 285 ~ 295
5. [학술지(정기간행물)] - Eskelinen EL / 2009 / Autophagy: a lysosomal degradation pathway with a central role in health and disease / Biochim Biophys Acta 1793 (4) : 664 ~ 673
6. [학술지(정기간행물)] - Kabeya Y / 2000 / LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing / EMBO J 19 (21) : 5720 ~ 5728
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