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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.9
- 수록면
- 162 - 170 (9page)
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Purpose: Sodium-4-phenylbutyrate (Na-4-PB) is an analogue of phenylacetate, which is a well-known redifferentiating agent. In vitro and in vivo studies on this agent have been done and the clinical relevance of Na-4-PB has been studied in other malignancies, but not in thyroid cancer. We investigated the effect of Na-4-PB on cell proliferation and differentiation in thyroid cancer cell lines.
Methods: We used 5 thyroid cancer cell lines: TPC-1, FTC-133, FTC-236, FTC-238 and XTC-1. MTT assay and flowcytometry were used to measure the agent’s antiproliferative effects and the cell cycle change. We evaluated the PPARγ expression via western blotting and the mRNA expressions of NIS, Tg and CD 97 were determined by performing RT-PCR. Troglitazone, a potent PPARγ agonist, was used in combined treatment with Na-4-PB.
Results: Na-4-PB inhibited cell proliferation in a dose and time dependent manner in all 5 thyroid cancer cell lines. By performing flowcytometry in the FTC-133 and TPC-1 cell lines, we identified that the antiproliferative effect of Na-4-PB was associated with an increased apoptotic cell population. Treatment with Na-4-PB upregulated the PPARγ expression, but the combined treatment of Na-4-PB with troglitazone did not seem to be synergistic for the antiproliferative effect. Treatment with Na-4-PB downregulated the CD97 mRNA expression and it upregulated the NIS and Tg mRNA expressions in both the FTC-133 and TPC-1 cell lines.
Conclusion: Na-4-PB inhibited thyroid cancer cell proliferation by inducing apoptosis in a dose dependent manner. Treatment with Na-4-PB increased the expression of PPARγ and it upregulated such differentiation markers as NIS and Tg, and it downregulated CD97, a dedifferentiation marker. Na-4-PB should be further evaluated as a new potential therapeutic agent for patients with thyroid cancer.
Methods: We used 5 thyroid cancer cell lines: TPC-1, FTC-133, FTC-236, FTC-238 and XTC-1. MTT assay and flowcytometry were used to measure the agent’s antiproliferative effects and the cell cycle change. We evaluated the PPARγ expression via western blotting and the mRNA expressions of NIS, Tg and CD 97 were determined by performing RT-PCR. Troglitazone, a potent PPARγ agonist, was used in combined treatment with Na-4-PB.
Results: Na-4-PB inhibited cell proliferation in a dose and time dependent manner in all 5 thyroid cancer cell lines. By performing flowcytometry in the FTC-133 and TPC-1 cell lines, we identified that the antiproliferative effect of Na-4-PB was associated with an increased apoptotic cell population. Treatment with Na-4-PB upregulated the PPARγ expression, but the combined treatment of Na-4-PB with troglitazone did not seem to be synergistic for the antiproliferative effect. Treatment with Na-4-PB downregulated the CD97 mRNA expression and it upregulated the NIS and Tg mRNA expressions in both the FTC-133 and TPC-1 cell lines.
Conclusion: Na-4-PB inhibited thyroid cancer cell proliferation by inducing apoptosis in a dose dependent manner. Treatment with Na-4-PB increased the expression of PPARγ and it upregulated such differentiation markers as NIS and Tg, and it downregulated CD97, a dedifferentiation marker. Na-4-PB should be further evaluated as a new potential therapeutic agent for patients with thyroid cancer.
목차
서론
방법
결과
고찰
REFERENCES
참고문헌 (30)
참고문헌 신청
1. [학술지(정기간행물)] - Lea MA / 2004 / Inhibition of cell proliferation by potential peroxisome proliferator-activated receptor (PPAR) gamma agonists and antagonists / Anticancer Res 24 : 2765 ~ 2771
2. [학술지(정기간행물)] - Park JW / 2004 / Redifferentiation therapy for thyroid cancer / Surg Clin North Am 84 : 921 ~ 943
3. [학술지(정기간행물)] - Haugen BR / 2004 / Redifferentiation therapy in advanced thyroid cancer / Curr Drug Targets Immune Endocr Metabol Disord 4 : 175 ~ 180
4. [학술지(정기간행물)] - Brusilow SW / 1984 / Treatment of episodic hyperammonemia in children with inborn errors of urea synthesis / N Engl J Med 310 : 1630 ~ 1634
5. [학술지(정기간행물)] - Samid D / 1992 / Phenylacetate: a novel nontoxic inducer of tumor cell differentiation / Cancer Res 52 : 1988 ~ 1992
2. [학술지(정기간행물)] - Park JW / 2004 / Redifferentiation therapy for thyroid cancer / Surg Clin North Am 84 : 921 ~ 943
3. [학술지(정기간행물)] - Haugen BR / 2004 / Redifferentiation therapy in advanced thyroid cancer / Curr Drug Targets Immune Endocr Metabol Disord 4 : 175 ~ 180
4. [학술지(정기간행물)] - Brusilow SW / 1984 / Treatment of episodic hyperammonemia in children with inborn errors of urea synthesis / N Engl J Med 310 : 1630 ~ 1634
5. [학술지(정기간행물)] - Samid D / 1992 / Phenylacetate: a novel nontoxic inducer of tumor cell differentiation / Cancer Res 52 : 1988 ~ 1992
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