Therapeutic potential of adipose tissue-derived stem cells for liver failure according to the transplantation routes

Say-June Kim; Ki Cheol Park; Jung Uee Lee; Kwan-Ju Kim; Dong-Goo Kim

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자료유형: 학술저널

저자정보: Say-June Kim (The Catholic University) Ki Cheol Park (The Catholic University) Jung Uee Lee (The Catholic University) Kwan-Ju Kim (The Catholic University) Dong-Goo Kim (The Catholic University)

저널정보: 대한외과학회 Annals of Surgical Treatment and Research 대한외과학회지 Vol.81 No.3

발행연도: 2011.9

수록면: 176 - 186 (11page)

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Purpose: Even though adipose tissue-derived stem cells (ADSCs) have been spotlighted as a possible alternative for liver transplantation in an experimental setting, the mechanism by which ADSCs improve liver dysfunction remains poorly characterized. The objective of this study was to evaluate the therapeutic ability of undifferentiated ADSCs, and find a few clues on how ADSCs alleviate liver damage by comparing the transplantation routes. Methods: In vitro generated human ADSCs were checked for surface markers and stage-specific genes for characterization. Afterwards, they were transplanted into C57BL/6 mice with CCl4-induced liver injury. The transplantations were made via tail vein, portal vein, and direct liver parenchymal injection. At 1 and 3 post-transplantation days, serum biochemical parameters and/or liver specimens were evaluated. Results: We have shown here that ADSCs have the characteristics of mesenchymal stem cells, and belong to endodermal and/or early hepatic differentiation stage. After transplantation into the mice with acute liver failure, markers of liver injury, such as alanineaminotransferase, aspartateaminotransferase, as well as ammonia, decreased. Of these transplantation routes, transplantation via tail vein rendered the most prominent reduction in the biochemical parameters. Conclusion: Undifferentiated ADSCs have the ability to improve hepatic function in mice with acute liver injury. Moreover, our transplantation route study supports the theory that ADSCs in systemic circulation can exert endocrine or paracrine effects to ameliorate the injured liver.

#Adipose tissue-derived stem cell #Mesenchymal stem cells #Acute liver injury #Stem cell transplantation

참고문헌 (30)

참고문헌 신청

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