지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.2
- 수록면
- 117 - 127 (11page)
이용수
초록· 키워드
오류제보하기
Purpose: The lack of reliable in vitro infection systems or convenient animal models has hindered the progress of hepatitis B virus (HBV) research and the development of new treatment options. We established an in vitro model of hepatitis B, using recombinant HBV encoding baculovirus, which provided HBV replication and antigens expression in HepG2 cells. The objectives of this study were to characterize the magnitude of HBV expression and the level of replication obtainable in HepG2 cells, to establish the optimum infection and culture conditions of HBV expression and replication.
Methods: Replication of a competent HBV genome encoding the baculovirus, RC-HBV-Bac, was generated for delivering the HBV genome to HepG2 cells. HBV replication and antigens expression were determined in relation to the infection and culture conditions.
Results: In RC-HBV-Bac infected HepG2 cells, HBsAg, HBeAg and HBcAg were expressed in the cytoplasm and nuclei, and secreted into the medium. HBV replication was evidenced by the presence of a replication complex and covalently closed circular (ccc) DNA in the cytoplasmic fraction of infected cells. The level of HBV expression was directly proportional to the multiplication of RC-HBV-Bac infection. Polyethylene glycol was able to enhance the infection efficiency of the baculovirus to HepG2 cells. High levels of HBV replication were achieved under culture conditions supplemented with dimethyl sulfoxide and a low serum concentration.
Conclusion: This in vitro model of hepatitis B, generated by baculovirus gene delivery, represents a simple and flexible system for the study of HBV replication and drug testing.
Methods: Replication of a competent HBV genome encoding the baculovirus, RC-HBV-Bac, was generated for delivering the HBV genome to HepG2 cells. HBV replication and antigens expression were determined in relation to the infection and culture conditions.
Results: In RC-HBV-Bac infected HepG2 cells, HBsAg, HBeAg and HBcAg were expressed in the cytoplasm and nuclei, and secreted into the medium. HBV replication was evidenced by the presence of a replication complex and covalently closed circular (ccc) DNA in the cytoplasmic fraction of infected cells. The level of HBV expression was directly proportional to the multiplication of RC-HBV-Bac infection. Polyethylene glycol was able to enhance the infection efficiency of the baculovirus to HepG2 cells. High levels of HBV replication were achieved under culture conditions supplemented with dimethyl sulfoxide and a low serum concentration.
Conclusion: This in vitro model of hepatitis B, generated by baculovirus gene delivery, represents a simple and flexible system for the study of HBV replication and drug testing.
목차
서론
방법
결과
고찰
결론
REFERENCES
참고문헌 (22)
참고문헌 신청
1. [학술지(정기간행물)] - 박희승 / 2006 / 만성 B형간염에서 라미부딘 치료에 의한 혈청 HBV DNA치의 변화와 Viral Breakthrough의 관련성 / Clinical and Molecular Hepatology 12 (2) : 173 ~ 183
2. [학술지(정기간행물)] - 김연수 / 2006 / HBsAg 음성인 간질환 환자에서 간조직내 HBV DNA의 검출 / Clinical and Molecular Hepatology 12 (2) : 201 ~ 208
3. [학술대회논문] - Doong SL / 1991 / Inhibition of the replication of hepatitis B virus in vitro by 2',3'-dideoxy-3'-thiacytidine and related analogues / Proc Natl Acad Sci USA 88 : 8495 ~ 8499
4. [학술지(정기간행물)] - Aoki-Sei S / 1991 / In vitro inhibition of hepatitis B virus replication by 2',3'-dideoxyguanosine, 2',3'-dideoxyinosine, and 3'-azido-2',3'-dideoxythymidine in 2.2.15 (PR) cells / J Infect Dis 164 : 843 ~ 851
5. [학술지(정기간행물)] - Delaney WE 4th / 1999 / Use of the hepatitis B virus recombinant baculovirus-HepG2 system to study the effects of (-)-beta-2',3'-dideoxy-3'-thiacytidine on replication of hepatitis B virus and accumulation of covalently closed circular DNA / Antimicrob Agents Chemother 43 : 2017 ~ 2026
2. [학술지(정기간행물)] - 김연수 / 2006 / HBsAg 음성인 간질환 환자에서 간조직내 HBV DNA의 검출 / Clinical and Molecular Hepatology 12 (2) : 201 ~ 208
3. [학술대회논문] - Doong SL / 1991 / Inhibition of the replication of hepatitis B virus in vitro by 2',3'-dideoxy-3'-thiacytidine and related analogues / Proc Natl Acad Sci USA 88 : 8495 ~ 8499
4. [학술지(정기간행물)] - Aoki-Sei S / 1991 / In vitro inhibition of hepatitis B virus replication by 2',3'-dideoxyguanosine, 2',3'-dideoxyinosine, and 3'-azido-2',3'-dideoxythymidine in 2.2.15 (PR) cells / J Infect Dis 164 : 843 ~ 851
5. [학술지(정기간행물)] - Delaney WE 4th / 1999 / Use of the hepatitis B virus recombinant baculovirus-HepG2 system to study the effects of (-)-beta-2',3'-dideoxy-3'-thiacytidine on replication of hepatitis B virus and accumulation of covalently closed circular DNA / Antimicrob Agents Chemother 43 : 2017 ~ 2026
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
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UCI(KEPA) : I410-ECN-0101-2013-514-002674480