지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objective: To evaluate the immune-stimulatory potential of extracts of Broussonetia kazinoki Siebold (BK) on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice.
Material and Methods: C57BL/6 mice were immunized intraperitoneally with OVA/alum (100 ㎍/200 ㎍) on days 1, 8, and 15. BK (100, 300 or 1000 ㎎/㎏) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Further, the effects of BK on expression of cytokine mRNA in OVA-immunized mice splenocytes were evaluated by RT-PCR analysis.
Results: BK significantly enhanced OVA-, LPS-, and Con A-induced splenocyte proliferation in OVA-immunized mice (p＜0.01). BK also significantly enhanced total IgM and OVA-specific IgG1 levels in plasma compared with the OVA control group. Moreover, BK up-regulated significantly the expression of mRNA level of IL-2 and IFN-γ in splenocytes.
Conclusions: BK has immune-stimulating activity in an OVA-immunized mouse model system, enhancing the Th1 immune response. BK showed no cytotoxicity in this system, suggesting that BK may be a safe and effective adjuvant in humans.
Material and Methods: C57BL/6 mice were immunized intraperitoneally with OVA/alum (100 ㎍/200 ㎍) on days 1, 8, and 15. BK (100, 300 or 1000 ㎎/㎏) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Further, the effects of BK on expression of cytokine mRNA in OVA-immunized mice splenocytes were evaluated by RT-PCR analysis.
Results: BK significantly enhanced OVA-, LPS-, and Con A-induced splenocyte proliferation in OVA-immunized mice (p＜0.01). BK also significantly enhanced total IgM and OVA-specific IgG1 levels in plasma compared with the OVA control group. Moreover, BK up-regulated significantly the expression of mRNA level of IL-2 and IFN-γ in splenocytes.
Conclusions: BK has immune-stimulating activity in an OVA-immunized mouse model system, enhancing the Th1 immune response. BK showed no cytotoxicity in this system, suggesting that BK may be a safe and effective adjuvant in humans.
목차
Introduction
Methods & Materials
Results
Discussion
Acknowledgements
References
참고문헌 (21)
참고문헌 신청
1. [학술지(정기간행물)] - Livingston PO / 1994 / Phase 1 trial of immunological adjuvant QS-21 with a GM2 ganglioside-keyhole limpet haemocyanin conjugate vaccine in patients with malignant melanoma / Vaccine 12 (14) : 1275 ~ 1280
2. [학술지(정기간행물)] - Cox JC / 1997 / Adjuvants-a classification and review of their modes of action / Vaccine 15 (3) : 48 ~ 56
3. [학술지(정기간행물)] - Pascual DM / 2006 / Aduvants present regulatory challenges / Vaccine 24 (S2) : S88 ~ S89
4. [학술지(정기간행물)] - HogenEsch H / 2002 / Mechanisms of stimulation of the immune response by aluminum adjuvants / Vaccine 20 (S3) : S34 ~ S39
5. [학술지(정기간행물)] - Relyveld EH / 1998 / Rational approaches to reduce reactions man to vaccines containing tetanus and diphtheria toxoids / Vaccine 16 (9-10) : 1016 ~ 1023
2. [학술지(정기간행물)] - Cox JC / 1997 / Adjuvants-a classification and review of their modes of action / Vaccine 15 (3) : 48 ~ 56
3. [학술지(정기간행물)] - Pascual DM / 2006 / Aduvants present regulatory challenges / Vaccine 24 (S2) : S88 ~ S89
4. [학술지(정기간행물)] - HogenEsch H / 2002 / Mechanisms of stimulation of the immune response by aluminum adjuvants / Vaccine 20 (S3) : S34 ~ S39
5. [학술지(정기간행물)] - Relyveld EH / 1998 / Rational approaches to reduce reactions man to vaccines containing tetanus and diphtheria toxoids / Vaccine 16 (9-10) : 1016 ~ 1023
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UCI(KEPA) : I410-ECN-0101-2014-519-000281275