We present a novel method to selectively pattern nano-particles or biological materials on a substrate where locally hydrophilic regions could be formed on a super-hydrophobic surface. For the hydrophilic and super-hydrophobic heterogeneous surfaces, at first, the super-hydrophobic surface was prepared by using the atmospheric plasma treatment and selectively the layer could be removed forming any hydrophilic areas (e.g. dot arrays, grooved lines) on the substrate. The local hydrophilic region could be fabricated based on the chemical reaction between the super-hydrophobic surface and the Ethanol (or Butyl carbitol). We investigated the optimal condition of the selective formation of the hydrophilic region. We also could pattern tiny regions with the ethanol by using the electrohydrodynamic jet printing technology which allows us to print micro- to nano-scale geometry.