Increasing the shod-surface traction has been shown to lead to increased incidence of anterior cruciate ligament (ACL) injuries, however it remains unclear how these shoes may affect the knee kinematics and kinetics during single-leg landing. The purpose of this study was to determine whether shoe wear alters valgus moment and tibial internal rotation moment. Healthy male subjects were recruited and instructed to perform single-leg landing from 0.3-m heights in barefoot and shod conditions. Force-plates and motion-capture system were used to capture ground reaction force (GRF) and kinematics data respectively. Peak knee valgus moment (p<0.01) and peak tibial internal rotation moment (p<0.01) were higher during shod landing, compared to barefoot landing. In conclusion, this study has shown that shod condition was associated with biomechanical conditions that can increase the risk of ACL injury.