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논문 기본 정보

자료유형
학술저널
저자정보
Jwakyung Sung (RDA) Suyeon Lee (RDA) Yejin Lee (RDA) Rogyoung Kim (Kangwon National University) Juyoung Lee (RDA) Jongsik Lee (RDA) Yongsik Ok (Kangwon National University)
저널정보
한국토양비료학회 한국토양비료학회지 한국토양비료학회지 제45권 제6호
발행연도
2012.12
수록면
910 - 919 (10page)

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초록· 키워드

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The role of inorganic nitrogen assimilation in the production of amino acids, organic acids and soluble sugars is one of the most important biochemical processes in plants, and, in order to achieve normally, nitrate uptake and assimilation is essential. For this reason, the characterization of nitrate assimilation and metabolite composition from leaves, roots and xylem sap of tomato (Solanum lycopersicum) was investigated under different nitrate levels in media. Tomato plants were grown hydroponically in liquid culture under five different nitrate regimes: deficient (0.25 and 0.75 mM NO<sub>3</sub> -), normal (2.5 mM NO<sub>3</sub> -) and excessive (5.0 and 10.0 mM NO<sub>3</sub> -). All samples, leaves, roots and xylem sap, were collected after 7 and 14 days after treatment. The levels of amino acids, soluble sugars and organic acids were significantly decreased by N-deficiency whereas, interestingly, they remained higher in xylem sap as compared with N-normal and -surplus. The N-excessive condition did not exert any significant changes in metabolites composition, and thus their levels were similar with N-normal. The gene expression and enzyme activity of nitrate reductase (NR), nitrite reductase (NIR) and glutamine synthetase (GS) were greatly influenced by nitrate. The data presented here suggest that metabolites, as a signal messenger, existed in xylem sap seem to play a crucial role to acquire nitrate, and, in addition, an increase in α-ketoglutarate pathway-derived amino acids under N-deficiency may help to better understand plant C/N metabolism.

목차

Introduction
Materials and Method
Results
Discussion
References

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UCI(KEPA) : I410-ECN-0101-2014-520-003118949